Index-agnostic oblique plane light sheet microscopy of centimetre-scale cleared tissues at subcellular resolution
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We present cleared-tissue direct-view oblique plane microscopy (CtDvOPM), which enables optically sectioned subcellular resolution imaging of centimetre-scale tissues at high throughput over the full range of clearing media refractive indices ( n = 1.33–1.56). CtDvOPM can image conventionally-mounted expanded, aqueous or non-aqueous cleared tissue samples at up to 2 µm lateral by 14 µm axial resolution over a 10.1 mm × 25.0 mm × 6.8 mm ( x × y × z ) sample volume without image tiling, at up to 400 million voxels per second.
