Protocol for 3D digital dynamic histomorphometry of bone via time-lapse registration of serial microCT scans
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Serial in vivo microCT enables quantification of dynamic bone remodeling by capturing formation and resorption over time. This protocol uses Dragonfly to register pre- and post-intervention scans of rodent long bones through an intuitive drag-and-click workflow that requires no coding expertise. Rigid registration enables spatial mapping and quantification of formed, resorbed, and quiescent cortical and trabecular bone at periosteal and endosteal surfaces across multiple tibial regions, providing a non-destructive and complementary alternative to classic, fluorochrome-based dynamic histomorphometry.
