Two-dye-imager DNA-PAINT enables volumetric nanoscopy of expanded cells
Discuss this preprint
Start a discussion What are Sciety discussions?Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
Refined single-molecule localization microscopy methods demonstrated superior localization precisions on isolated sample but remain limited by labeling density and imaging speed in cells. Here we combine expansion microscopy (ExM) with two-dye-imager (TDI)-DNA-PAINT to resolve fine molecular details of protein assemblies in ∼8-fold expanded cells with nanometer resolution. Using lattice light-sheet (LLS) microscopy, Ex-TDI-DNA-PAINT provides a robust platform for three-dimensional (3D) volumetric nanoscopy of the molecular organization of cells.
