Unlocking the potential of Gordonia rubripertincta in syngas fermentation for carbon monoxide bioconversion into carotenoids
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Fermentation of C1 gases is an emerging technology where waste gases are bio converted into value-added products. This study navigates the gas fermentation potential of Gordonia rubripertincta to produce carotenoids. The crucial carbon monoxide dehydrogenase (CODH) enzyme, necessary for gas uptake by the microbe, was found to be present in G. rubripertincta through blastp on NCBI website. The organism was then used for gas fermentation experiments in a continuous stirred tank reactor (CSTR) in different modes of reactor operation resulting in the production of about 500 mg pigment/g WCW (wet cell weight). Two important reactor parameters, molybdenum content and pH, were optimized for enhanced carotenoid production. Overall, G. rubripertincta was observed to be an efficient candidate organism for C1 gas fermentation.
KEY HIGHLIGHTS
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Gordonia rubripertincta synthesises aerobic carbon monoxide dehydrogenase enzyme.
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It is a potential gas fermenting microbe that gives carotenoids as product.
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The gas uptake efficiency of the microbe is more in fed-batch discontinued mode.
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In FB-D, the resultant carotenoids are 500 + 9 mg/g wet cell weight (WCW).
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Mo/pH of 20 mg/7.0 resulted in highest carotenoids, i.e., 134 + 41 mg/g WCW.
