3-D Ontogenetic Staging Atlas of the Epaulette Shark Hemiscyllium ocellatum, a Laboratory Model for Shark Development

Chondrichthyans (cartilaginous fishes) form the sister group to osteichthyans (bony fishes) and therefore occupy a key phylogenetic position for comparative studies of early vertebrate evolution. Despite their importance, chondrichthyan development remains understudied relative to established model systems such as mouse, chick, and zebrafish, in part because of limited embryo accessibility and the lack of standardized laboratory resources for rearing. Here, we present the epaulette shark Hemiscyllium ocellatum , a small, oviparous shark as a tractable laboratory system for studying shark development. We provide an overview of epaulette shark husbandry requirements and generate a comprehensive micro-computed tomography imaging series spanning embryonic development through hatching. This dataset provides a three-dimensional anatomical atlas of development for a representative chondrichthyan species. By preserving whole embryos in three dimensions, micro-CT imaging enables developmental morphologies to be visualized at high resolution and in near-native anatomical context. Together with the recently published epaulette shark genome, this developmental atlas helps establish the Epaulette shark for comparative anatomical, developmental, and genomic studies.