Ubiquitin-proteasome pathway annotation in Diaphorina citri can reveal potential targets for RNAi-based pest management

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Ubiquitination is an ATP-dependent process that targets proteins for degradation by the proteasome. Here, we annotated 15 genes from the ubiquitin-proteasome pathway in the Asian citrus psyllid, Diaphorina citri. This psyllid vector has come to prominence in the last decade owing to its role in the transmission of the devastating bacterial pathogen, Candidatus Liberibacter asiaticus (CLas). Infection of citrus crops by this pathogen causes Huanglongbing (HLB), or citrus greening disease, and results in the eventual death of citrus trees. The identification and correct annotation of these genes in D. citri will be useful for functional genomic studies to aid the development of RNAi-based management strategies aimed at reducing the spread of HLB. Investigating the effects of CLas infection on the expression of ubiquitin-proteasome pathway genes may provide new information about the role these genes play in the acquisition and transmission of CLas by D. citri.

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  1. Abstract

    This paper has been published in GigaByte as part of the Asian citrus psyllid community annotation series. https://doi.org/10.46471/GIGABYTE_SERIES_0001.

    The CC-BY 4.0 peer reviews are as follows:

    Reviewer 1. Mary Ann Tuli Are all data available and do they match the descriptions in the paper?

    Yes. As with the other manuscripts, OGS v3 is mentioned, but this is not get available from the CGEN. The data underlying Fig 4 and Fig5 are available.

    This manuscript is a comprehensive description of the manual curation of the ubiquitin proteasome pathway gene, with clear aims and methodology.

  2. Ubiquitination

    **Reviewer 2. Subhas Hajeri **

    The manuscript is well written. Even though the authors could not find a major impact of CLas infection on the annotated, subset of ubiquitin-proteasome genes but the negative data is also equally important for further understanding of pathways and developing better RNAi targets.

    I would like to recommend acceptance of the manuscript as is.