Annotation of glycolysis, gluconeogenesis, and trehaloneogenesis pathways provide insight into carbohydrate metabolism in the Asian citrus psyllid

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Citrus greening disease is caused by the pathogen Candidatus Liberibacter asiaticus and transmitted by the Asian citrus psyllid, Diaphorina citri. No curative treatment or significant prevention mechanism exists for this disease, which causes economic losses from reduced citrus production. A high-quality genome of D. citri is being manually annotated to provide accurate gene models to identify novel control targets and increase understanding of this pest. Here, we annotated 25 D. citri genes involved in glycolysis and gluconeogenesis, and seven in trehaloneogenesis. Comparative analysis showed that glycolysis genes in D. citri are highly conserved but copy numbers vary. Analysis of expression levels revealed upregulation of several enzymes in the glycolysis pathway in the thorax, consistent with the primary use of glucose by thoracic flight muscles. Manually annotating these core metabolic pathways provides accurate genomic foundation for developing gene-targeting therapeutics to control D. citri.

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  1. Abstract

    A version of this preprint has been published in the Open Access journal GigaByte (see paper, where the paper and peer reviews are published openly under a CC-BY 4.0 license.

    **Reviewer 1. Mary Ann Tuli **

    Are all data available and do they match the descriptions in the paper? No. The paper states "The D. citri genome assembly (v3), OGS (v3) and transcriptomes are accessible on the portal" I believe v2 is available, not v3 yet.

    Additional Comments The paper states "The gene models will be part of an updated official gene set (OGS) for D. citri that will be submitted to NCBI." Until these models are available in NCBI their reuse is limited.

    Recommendation: Minor Revision

  2. Citrus greening disease

    **Reviewer 2. Xinyu Li **

    In the paper entitled “Annotation of glycolysis, gluconeogenesis, and trehaloneogenesis pathways provide insight into carbohydrate metabolism in the Asian citrus psyllid”, the authors conducted a high quality annotation of genes involved in glycolysis, gluconeogenesis, and trehaloneogenesis in Diaphorina citri genome, which provided the bases to develop gene-targeting therapeutics for this important pest species.

    The MS is well-written, and the analyses are clear and proper. I found some minor concerns that should be addressed.

    In the first paragraph of Page 10, the authors used cross symbol and the asterisk in the sentence “The number of genes identified in glycolysis….from NCBI, OrthoDB, and Flybase.”. However, the cross symbol and the asterisk are used without any explanation or citation. I suggest to cite the Appendix the authors referred to or add an explanation to make it clearer.

    In Conclusion part, on Page 15, the authors stated “Expression analysis of the genes annotated in the carbohydrate metabolism pathways identified differences related to life stage, sex and tissue.”. But what are the differences are not mentioned here. I think it would be better to summarize the key/predominant differences about gene expression in the carbohydrate metabolism pathways.

    In addition, it is interesting that the gene expression related with carbohydrate metabolism is sexually different in the Asian citrus psyllid. Is it common in insects or existed in some specific groups?