Boosting of the SARS-CoV-2–Specific Immune Response after Vaccination with Single-Dose Sputnik Light Vaccine
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Abstract
Despite measures taken world-wide, the coronavirus disease 2019 (COVID-19) pandemic continues. Because efficient antiviral drugs are not yet widely available, vaccination is the best option to control the infection rate. Although this option is obvious in the case of COVID-19–naive individuals, it is still unclear when individuals who have recovered from a previous SARS-CoV-2 infection should be vaccinated and whether the vaccination raises immune responses against the coronavirus and its novel variants. In this study, we collected peripheral blood from 84 healthy human donors of different COVID-19 status who were vaccinated with the Sputnik Light vaccine and measured the dynamics of the Ab and T cell responses, as well as the virus-neutralizing activity (VNA) in serum, against two SARS-CoV-2 variants, B.1.1.1 and B.1.617.2. We showed that vaccination of individuals previously exposed to the virus considerably boosts the existing immune response. In these individuals, receptor-binding domain (RBD)–specific IgG titers and VNA in serum were already elevated on the 7th day after vaccination, whereas COVID-19–naive individuals developed the Ab response and VNA mainly 21 d postvaccination. Additionally, we found a strong correlation between RBD-specific IgG titers and VNA in serum, and according to these data vaccination may be recommended when the RBD-specific IgG titers drop to 142.7 binding Ab units/ml or below. In summary, the results of the study demonstrate that vaccination is beneficial for both COVID-19–naive and recovered individuals, especially since it raises serum VNA against the B.1.617.2 variant, one of the five SARS-CoV-2 variants of concern.
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SciScore for 10.1101/2021.10.26.21265531: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Ethics: This study was approved by the Moscow City Ethics Committee of the Research Institute of the Organization of Health and Healthcare Management and performed according to the Helsinki Declaration.
Consent: All participants provided their written informed consent.Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources The plates were washed twice with PBS, then washed twice with PBS containing 0.05% Tween-20, and incubated with biotinylated anti-human IFNγ detection antibody for 2 h at RT. anti-human IFNγsuggested: NoneSoftware and Algorithms Sentences Resources SARS-CoV-2–specific … SciScore for 10.1101/2021.10.26.21265531: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Ethics: This study was approved by the Moscow City Ethics Committee of the Research Institute of the Organization of Health and Healthcare Management and performed according to the Helsinki Declaration.
Consent: All participants provided their written informed consent.Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources The plates were washed twice with PBS, then washed twice with PBS containing 0.05% Tween-20, and incubated with biotinylated anti-human IFNγ detection antibody for 2 h at RT. anti-human IFNγsuggested: NoneSoftware and Algorithms Sentences Resources SARS-CoV-2–specific antibodies and virus neutralizing activity in serum: Titers of the immunoglobulins G (IgGs) specific to the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) protein were analyzed in serum using the automated ARCHITECT i1000SR analyzer with compatible reagent kit (Abbott, USA) according to the manufacturer’s standard protocol. Abbottsuggested: (Abbott, RRID:SCR_010477)Statistical analysis: Statistical analysis was performed with the Python3 programming language with numpy, scipy and pandas packages. Python3suggested: Nonenumpysuggested: (NumPy, RRID:SCR_008633)scipysuggested: (SciPy, RRID:SCR_008058)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04741061 Recruiting Study to Evaluate Efficacy, Immunogenicity and Safety of the… NCT04898140 Recruiting The Evaluation of Cellular and Humoral Immunity to COVID-19 … Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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