Angiotensin-converting Enzyme 2, a SARS-CoV-2 Receptor, Is Upregulated by Interleukin 6 through STAT3 Signaling in Synovial Tissues
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SciScore for 10.1101/2020.05.26.115261: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Study design: This study was approved by the clinical ethics committees of Hiroshima University Hospital
Consent: Synovial tissues were collected from 16 rheumatoid arthritis (RA) patients that fulfilled the classification criteria of the 1987 American College of Rheumatology (Arthritis and Rheum. 1988; 31: 315–324) and underwent total joint replacement, after obtaining informed and signed consent forms.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Following deparaffinization and antigen retrieval in Tris-EDTA buffer (pH 9.0), the specimens were … SciScore for 10.1101/2020.05.26.115261: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Study design: This study was approved by the clinical ethics committees of Hiroshima University Hospital
Consent: Synovial tissues were collected from 16 rheumatoid arthritis (RA) patients that fulfilled the classification criteria of the 1987 American College of Rheumatology (Arthritis and Rheum. 1988; 31: 315–324) and underwent total joint replacement, after obtaining informed and signed consent forms.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Following deparaffinization and antigen retrieval in Tris-EDTA buffer (pH 9.0), the specimens were first incubated with primary antibodies (anti-ACE2, rabbit polyclonal, Bioss Antibodies Inc., Woburn, MA, USA), and then with the AP-conjugated secondary antibodies. anti-ACE2suggested: NoneSoftware and Algorithms Sentences Resources RT-qPCR using Brilliant II SYBR® Green QPCR Master Mix (Agilent, Santa Clara, CA, USA) was outperformed on CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA). Bio-Rad Laboratoriessuggested: (Bio-Rad Laboratories, RRID:SCR_008426)Data were processed and analyzed using the GraphPad Prism 8 software (Graph Pad Software Inc., GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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