Angiotensin-converting Enzyme 2, a SARS-CoV-2 Receptor, Is Upregulated by Interleukin 6 through STAT3 Signaling in Synovial Tissues

  1. Sho Mokuda
  2. Tadahiro Tokunaga
  3. Junya Masumoto
  4. Eiji Sugiyama

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Abstract

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  1. Version published to 10.3899/jrheum.200547
  2. ScreenIT

    SciScore for 10.1101/2020.05.26.115261: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: Study design: This study was approved by the clinical ethics committees of Hiroshima University Hospital
    Consent: Synovial tissues were collected from 16 rheumatoid arthritis (RA) patients that fulfilled the classification criteria of the 1987 American College of Rheumatology (Arthritis and Rheum. 1988; 31: 315–324) and underwent total joint replacement, after obtaining informed and signed consent forms.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Following deparaffinization and antigen retrieval in Tris-EDTA buffer (pH 9.0), the specimens were first incubated with primary antibodies (anti-ACE2, rabbit polyclonal, Bioss Antibodies Inc., Woburn, MA, USA), and then with the AP-conjugated secondary antibodies.
    anti-ACE2
    suggested: None
    Software and Algorithms
    SentencesResources
    RT-qPCR using Brilliant II SYBR® Green QPCR Master Mix (Agilent, Santa Clara, CA, USA) was outperformed on CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA).
    Bio-Rad Laboratories
    suggested: (Bio-Rad Laboratories, RRID:SCR_008426)
    Data were processed and analyzed using the GraphPad Prism 8 software (Graph Pad Software Inc.,
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • No funding statement was detected.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.05.26.115261v1 on bioRxiv