Low Neutralizing Antibody Titers against the Mu Variant of SARS-CoV-2 in 31 BNT162b2 Vaccinated Individuals in Colombia
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Abstract
Global surveillance programs for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are showing the emergence of variants with mutations in the spike protein. Genomic and laboratory surveillance are important to determine if these variants may be more infectious or less susceptible to antiviral treatments and vaccine-induced antibodies. Three of the most predominant SARS-CoV-2 variants in Colombia during the epidemiological peaks of 2021 were isolated: Mu, a variant of interest; Gamma, a variant of concern; B.1.111, which lacks genetic markers associated with greater virulence. Microneutralization assays were performed by incubating 120 mean tissue culture infectious doses (TCID50) of each SARS-CoV-2 isolate with five two-fold serial dilutions of sera from 31 BNT162b2-vaccinated volunteers. The mean neutralization titer (MN50) was calculated by the Reed–Muench method. At the end of August, Mu represented 49% of coronavirus disease 2019 (COVID-19) cases in Colombia, followed by 25% of Gamma. In contrast, B.1.111 became almost undetectable. The evaluation of neutralizing antibodies suggests that patients vaccinated with BNT162b2 generate neutralizing antibody titers against the Mu variant at significantly lower concentrations relative to B.1.111 and Gamma. This study shows the importance of continuing surveillance programs of emerging variants, as well as the need to evaluate the neutralizing antibody response induced by other vaccines.
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SciScore for 10.1101/2021.11.19.21266552: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources Sera were first screened for the absence of IgG anti-nucleoprotein antibodies using a qualitative ELISA (ID Screen SARS-CoV-2-N IgG Indirect, ID Vet). anti-nucleoproteinsuggested: Nonesubsequently, to determine the concentration of anti-Spike IgG antibodies, samples were tested with the SARS-CoV-2 IgG assay (sCOVG) on the ADVIA Centaur XPT platform (Siemens) using the kit cut-off value (reactive ≥1.0 U/mL); the results were expressed in binding antibody units per milliliter (BAU)/mL using the conversion factor of 21.8 … SciScore for 10.1101/2021.11.19.21266552: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources Sera were first screened for the absence of IgG anti-nucleoprotein antibodies using a qualitative ELISA (ID Screen SARS-CoV-2-N IgG Indirect, ID Vet). anti-nucleoproteinsuggested: Nonesubsequently, to determine the concentration of anti-Spike IgG antibodies, samples were tested with the SARS-CoV-2 IgG assay (sCOVG) on the ADVIA Centaur XPT platform (Siemens) using the kit cut-off value (reactive ≥1.0 U/mL); the results were expressed in binding antibody units per milliliter (BAU)/mL using the conversion factor of 21.8 determined by the manufacturer based on WHO first standard 20/136 [11]. anti-Spike IgGsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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