Seroprevalence of Anti-SARS-CoV-2 Antibodies in Blood Donors from Nuevo Leon State, Mexico, during 2020: A Retrospective Cross-Sectional Evaluation
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Abstract
The progression and distribution of the SARS-CoV-2 pandemic are continuously changing over time and can be traced by blood donors’ serological survey. Here, we investigated the seroprevalence of anti-SARS-CoV-2 antibodies in blood donors in Nuevo Leon, Mexico during 2020 as a strategy for the rapid evaluation of the spread of SARS-CoV-2 and asymptomatic case detection. We collected residual plasma samples from blood donors who attended two regional donation centers from January to December of 2020 to identify changes in anti-SARS-CoV-2 IgG prevalence. Plasma samples were analyzed on the Abbott Architect instrument using the commercial Abbott SARS-CoV-2 IgG chemiluminescent assay. We found a total of 99 reactive samples from 2068 analyzed plasma samples, resulting in a raw prevalence of 4.87%. Donors aged 18–49 years were more likely to be seropositive compared to those aged >50 years (p < 0.001). Weekly seroprevalence increased from 1.8% during the early pandemic stage to 27.59% by the end of the year. Prevalence was 1.46-fold higher in females compared to males. Case geographical mapping showed that Monterrey city recorded the majority of SARS-CoV-2 cases. These results show that there is a growing trend of seroprevalence over time associated with asymptomatic infection that is unnoticed under the current epidemiological surveillance protocols.
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SciScore for 10.1101/2020.11.28.20240325: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The research protocol was reviewed and approved by the institutional review board.
Consent: We used exclusively residual plasma or serum from routine laboratory diagnosis of blood donors; therefore, the need for informed consent was waived.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources We used ArcGIS v10.2.2 (ESRI, Redlands, CA, USA) to indicate the municipality of donors actual residence. ArcGISsuggested: (ArcGIS for Desktop Basic, RRID:SCR_011081)HIV-1/2), Hepatitis B Virus (HBV), and Trypanosoma cruzi (Chagas disease), using an … SciScore for 10.1101/2020.11.28.20240325: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The research protocol was reviewed and approved by the institutional review board.
Consent: We used exclusively residual plasma or serum from routine laboratory diagnosis of blood donors; therefore, the need for informed consent was waived.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources We used ArcGIS v10.2.2 (ESRI, Redlands, CA, USA) to indicate the municipality of donors actual residence. ArcGISsuggested: (ArcGIS for Desktop Basic, RRID:SCR_011081)HIV-1/2), Hepatitis B Virus (HBV), and Trypanosoma cruzi (Chagas disease), using an Architect i2000 SR analyzer (Abbott Diagnostics, Chicago, USA). Abbottsuggested: (Abbott, RRID:SCR_010477), Redmond, WA) and exported to SPSS (version 13.0 for Windows; SPSS Inc., Chicago, IL) for statistical analyses. SPSSsuggested: (SPSS, RRID:SCR_002865)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:As limitations of our study, first, we note that the seroprevalence estimated from this study may not reflect the true underlying proportion exposed to SARS-CoV-2 in our country because blood donors are not representative of the overall population; second, we did not test samples for virus neutralization and therefore the neutralizing activities of the detected IgG antibodies are unknown. Third, a rapid decline in antibody titers and pro-inflammatory cytokines may be a common feature of non-severe SARS-CoV-2 infection. It has been suggested that asymptomatic individuals may have a weaker immune response to SARS-CoV-2 infection in contrast to symptomatic [18]. At the time of this study, SARS-CoV-2 serological tests available only allow IgG analysis and we only observed past infections with at least two or three weeks of evolution; more recent infectious events may be unnoticed. This implies that the cases may be higher. It is important to consider that the apparent decrease in the titers may lead to false-negative results over subsequent months, a phenomenon not yet well determined. In this study, samples were collected during the 5 months after diagnosis of the first confirmed case by RT-qPCR in Nuevo Leon. For this reason, this study is unlikely to be hampered by a drop in SARS-CoV-2 antibody levels. On the other hand, it is important to consider that for future seroprevalence studies it will be important to carry out more studies to validate the lifespan of the antibodies a...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
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- No protocol registration statement was detected.
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