Early Humoral Response Correlates with Disease Severity and Outcomes in COVID-19 Patients

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1. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2020.09.21.20198309: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement

   Consent: Human subjects: Signed informed consent forms were obtained from all patients as per institutional ethical approvals obtained from the Unit of Biomedical Ethics in King Abdulaziz University Hospital (Reference No 245-20), the Institutional Review Board at the Ministry of Health, Saudi Arabia (IRB Numbers: H-02-K-076-0320-279 and H-02-K-076-0420-285), and the Global Center for Mass Gatherings Medicine, Saudi Arabia (GCMGM) (No. 20/03A). IRB: Human subjects: Signed informed consent forms were obtained from all patients as per institutional ethical approvals obtained from the Unit of Biomedical Ethics in King Abdulaziz University Hospital (Reference No …

   SciScore for 10.1101/2020.09.21.20198309: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	Consent: Human subjects: Signed informed consent forms were obtained from all patients as per institutional ethical approvals obtained from the Unit of Biomedical Ethics in King Abdulaziz University Hospital (Reference No 245-20), the Institutional Review Board at the Ministry of Health, Saudi Arabia (IRB Numbers: H-02-K-076-0320-279 and H-02-K-076-0420-285), and the Global Center for Mass Gatherings Medicine, Saudi Arabia (GCMGM) (No. 20/03A). IRB: Human subjects: Signed informed consent forms were obtained from all patients as per institutional ethical approvals obtained from the Unit of Biomedical Ethics in King Abdulaziz University Hospital (Reference No 245-20), the Institutional Review Board at the Ministry of Health, Saudi Arabia (IRB Numbers: H-02-K-076-0320-279 and H-02-K-076-0420-285), and the Global Center for Mass Gatherings Medicine, Saudi Arabia (GCMGM) (No. 20/03A).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After another washing, plates were incubated with HRP□conjugated goat anti□human IgG (H□+□L) or IgM antibodies (Jackson ImmunoResearch, West Grove, PA) for 1 h, washed again, and incubated with TMB (3,3’,5,5’-tetramethylbenzidine) substrate (KPL, Gaithersburg, MD) at room temperature for 30□min.	anti□human IgG suggested: None IgM suggested: None
   To completely remove any excess amount of the rVSV-ΔG/G*-luciferase, 15 ml of DMEM containing rabbit polyclonal anti VSV-G antibody were added to the cells monolayer and incubated for 24 h at 37°C in 5% CO2 humidified incubator.	anti VSV-G suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Cells: Baby Hamster kidney BHK-21/WI-2 cell line (Kerafast, EH1011) and African Green monkey kidney-derived Vero E6 cell line (ATCC, 1586) were cultured in Dulbecco’s modified essential medium (DMEM) contained 100 U/ml of penicillin, and 100 μg/ml of streptomycin and supplemented with 5 and 10% fetal bovine serum (FBS) in a 5% CO2 environment at 37°C.	BHK-21/WI-2 suggested: RRID:CVCL_HB78)
   Briefly, a T-175 tissue culture flask of BHK21/WI-2 cells were transfected with 46 μg of pcDNA expressing codon-optimized full-length SARS-CoV-2 S protein (GenBank accession number: MN908947) using Lipofectamine ™ 2000 transfection reagent (Invitrogen).	BHK21/WI-2 suggested: RRID:CVCL_HB78)
   Then, a 100 μl of the pseudovirus–serum mixtures were transferred onto Vero E6 cell monolayers and incubated at 37°C in a 5% CO2 humidified incubator for 24 h.	Vero E6 suggested: None
   Software and Algorithms
   Sentences	Resources
   Median Inhibitory Concentration (IC50) neutralization titers were determined using four-parameter logistic (4PL) curve in GraphPad Prism V8 software (GraphPad Co.).	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)
   Statistical analysis: Statistical analyses and graphical presentations were conducted with GraphPad Prism version 8.0 software (	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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2. Version published to 10.3390/v12121390

   Dec 4, 2020
3. 

   Version published to 10.1101/2020.09.21.20198309v1 on medRxiv

   Sep 23, 2020