Research Progress on the Development and Application of Cyclodextrin-Based Chromatographic Stationary Phases

This review systematically summarizes the novel preparation methods of cyclodextrin-based chromatographic stationary phases and their applications for chiral recognition in separation techniques such as capillary gas chromatography and high-performance liquid chromatography. Aiming at the current situation that enantiomers of chiral compounds present significant differences at the pharmacological, pharmacodynamic, and toxicological levels, the core value of chromatographic chiral separation technology in the field of drug discovery and development is emphasized. By analyzing the unique cavity structure and excellent stereoselective properties of cyclodextrins, the mechanism of their action as a chromatographic stationary phase was elaborated. Combined with the typical applications of different derivatized cyclodextrin stationary phases in drug analysis, environmental testing, and biological samples, the value and potential of cyclodextrin stationary phases in stereoisomer separation are systematically demonstrated.