Repellent Activity of DEET and Biont-Based Mosquito Repellents in the Chinese Market Against the Asian Long-Horned Tick, Haemaphysalis longicornis

To investigate the repellent efficacy of commercially available mosquito repellents in China against Haemaphysalis longicornis, four representative DEET-based repellents and biont-derived repellents were selected. This study utilized a modified circular filter paper method repellent testing device to establish an evaluation system for assessing the repellent effects of each product against three developmental stages of H. longicornis: larvae, nymphs, and adults. In our study, for DEET-based repellents, Johnson demonstrated the highest repellency against larvae within 1 h, with an average repellency rate exceeding 80.14%. Yamei and Johnson exhibited repellency rates more than 91.11% for nymphs within 1 h, surpassing the other two DEET products. Repellency rates of Longliqi and Xiaohuanxiong fluctuated between 80.95% and 100% at different time points. Yamei, Longliqi, and Johnson achieved 100% repellency to adults within 1 h, while Xiaohuanxiong showed slightly lower efficacy. The four biont-derived repellents showed significant variations in efficacy: larval-stage repellency ranged from 14.29% to 88.89%, nymphal-stage repellency from 57.89% to 100%, and adult-stage repellency from 50% to 79.49%. CaliforniaBaby exhibited the highest efficacy, comparable or superior to DEET-based products, whereas Longhu demonstrated the weakest repellency and poor persistence. We further conducted a persistence test for CaliforniaBaby and found that it maintained >75% repellency against H. longicornis for 6 h. This study provides scientific evidence for selecting tick repellents in practice, offers guidance for purchasing commercial biont-derived tick repellents, and serves as a reference for developing safer, more effective tick repellents.