Iota-Carrageenan Prevents the Replication of SARS-CoV-2 in a Human Respiratory Epithelium Cell Line in vitro

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1. Version published to 10.3389/fviro.2021.746824

   Dec 15, 2021
2. 

   ScreenIT

   Apr 29, 2021

   SciScore for 10.1101/2021.04.27.441512: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   The Calu-3 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Corning, NY, USA) containing 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).	Calu-3 suggested: KCLB Cat# 30055, RRID:CVCL_0609)
   Viral master seed stock was prepared using T175 flasks of Vero E6 cells.	Vero E6 suggested: RRID:CVCL_XD71)
   Tenfold dilutions of virus samples from …

   SciScore for 10.1101/2021.04.27.441512: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   The Calu-3 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Corning, NY, USA) containing 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).	Calu-3 suggested: KCLB Cat# 30055, RRID:CVCL_0609)
   Viral master seed stock was prepared using T175 flasks of Vero E6 cells.	Vero E6 suggested: RRID:CVCL_XD71)
   Tenfold dilutions of virus samples from Calu-3 cells were added to monolayers of 80% confluent Vero cells at 37°C for 1 h.	Vero suggested: None
   Software and Algorithms
   Sentences	Resources
   2.6 Statistical analysis: In the cellular viability assays, One-way ANOVA followed by Dunnett’s multiple comparisons test was performed, and in the infection assays, the results were analyzed statistically by two-way ANOVA, both using GraphPad Prism version 9.1.0, GraphPad Software, San Diego, California USA, https://www.graphpad.com.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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3. 

   Version published to 10.1101/2021.04.27.441512v1 on bioRxiv

   Apr 27, 2021