Combined Prophylactic and Therapeutic Use Maximizes Hydroxychloroquine Anti-SARS-CoV-2 Effects in vitro

  1. Nicola Clementi
  2. Elena Criscuolo
  3. Roberta Antonia Diotti
  4. Roberto Ferrarese
  5. Matteo Castelli
  6. Lorenzo Dagna
  7. Roberto Burioni
  8. Massimo Clementi
  9. Nicasio Mancini

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Abstract

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  1. Version published to 10.3389/fmicb.2020.01704
  2. ScreenIT

    SciScore for 10.1101/2020.03.29.014407: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    Cells, virus and antivirals: Vero E6 (Vero C1008, clone E6 – CRL-1586; ATCC) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with non-essential amino acids (NEAA), penicillin/streptomycin (P/S), Hepes buffer and 10% (v/v) Fetal bovine serum (FBS).
    Vero C1008
    suggested: ATCC Cat# CRL-1586, RRID:CVCL_0574)
    A clinical isolate hCoV-19/Italy/UniSR1/2020 (GISAID accession ID: EPI_ISL_413489) was isolated and propagated in Vero E6 cells, and viral titer was determined by 50% tissue culture infective dose (TCID50) and plaque assay for confirming the obtained titer.
    Vero E6
    suggested: None
    Software and Algorithms
    SentencesResources
    The quality of raw sequences obtained from MiSeq run was first checked using FastQC (v 0.11.5) (Babraham Bioinformatics).
    MiSeq
    suggested: (A5-miseq, RRID:SCR_012148)
    FastQC
    suggested: (FastQC, RRID:SCR_014583)
    The reads were aligned on reference sequence (GISAID accession ID: EPI_ISL_412973) using BWA-mem and rescued using Samtools alignment/Map (v 1.9) and bamtoFastq.
    BWA-mem
    suggested: (Sniffles, RRID:SCR_017619)
    Samtools
    suggested: (SAMTOOLS, RRID:SCR_002105)
    Finally, the contigs were generated using SPAdes (v 3.12.0).
    SPAdes
    suggested: (SPAdes, RRID:SCR_000131)
    Then, two-way ANOVA and Tukey’s multiple comparisons test were performed for the evaluation of Ct differences (GraphPad Prism 8).
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
    • No funding statement was detected.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.03.29.014407v1 on bioRxiv