CoVaccine HT™ Adjuvant Potentiates Robust Immune Responses to Recombinant SARS-CoV-2 Spike S1 Immunization
This article has been Reviewed by the following groups
Discuss this preprint
Start a discussion What are Sciety discussions?Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2020.07.24.220715: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable 2.1 Vaccination and Serum Collection: BALB/c mice (7-8 weeks of age, male and female) were immunised twice, three weeks apart, intramuscularly (IM) with 5 μg of SARS-CoV-2 Spike S1 (Sino Biological 40592-V05H) protein with or without adjuvants, or adjuvant alone, using an insulin syringe with a 29-gauge needle. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Detection antibodies were subclass specific goat anti-mouse polyclonal R-PE-conjugated antibodies (Southern Biotech) used at a 1:200 dilution. anti-mousesuggested…SciScore for 10.1101/2020.07.24.220715: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable 2.1 Vaccination and Serum Collection: BALB/c mice (7-8 weeks of age, male and female) were immunised twice, three weeks apart, intramuscularly (IM) with 5 μg of SARS-CoV-2 Spike S1 (Sino Biological 40592-V05H) protein with or without adjuvants, or adjuvant alone, using an insulin syringe with a 29-gauge needle. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Detection antibodies were subclass specific goat anti-mouse polyclonal R-PE-conjugated antibodies (Southern Biotech) used at a 1:200 dilution. anti-mousesuggested: NoneThe tests were set up in duplicates, and the costimulatory anti-CD28 antibody (0.1 μg/mL) was added to the cells during the incubation. anti-CD28suggested: NoneExperimental Models: Cell Lines Sentences Resources The serum-virus mixtures were added to a monolayer of confluent Vero E6 cells and incubated for 1 hour at 37°C in 5% CO2. Vero E6suggested: NoneExperimental Models: Organisms/Strains Sentences Resources 2.1 Vaccination and Serum Collection: BALB/c mice (7-8 weeks of age, male and female) were immunised twice, three weeks apart, intramuscularly (IM) with 5 μg of SARS-CoV-2 Spike S1 (Sino Biological 40592-V05H) protein with or without adjuvants, or adjuvant alone, using an insulin syringe with a 29-gauge needle. BALB/csuggested: NoneSoftware and Algorithms Sentences Resources Cut-offs were generated by determining the mean MFI values plus three standard deviations as determined by Microsoft Office Excel program Microsoft Office Excelsuggested: (Microsoft Excel, RRID:SCR_016137)Graphical representation of the data was done using Prism, Graphpad Software (San Diego, CA). Prismsuggested: (PRISM, RRID:SCR_005375)Graphpadsuggested: (GraphPad, RRID:SCR_000306)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
