Increased Plasma Heparanase Activity in COVID-19 Patients
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SciScore for 10.1101/2020.06.12.20129304: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement ( The local independent ethical committee approved the study protocol (CMO 2020-6344, CMO 2020-6359, CMO 2016-2923). Randomization Samples were randomly distributed over plates. Blinding not detected. Power Analysis not detected. Sex as a biological variable More men than women were included (Table 1). Table 2: Resources
Antibodies Sentences Resources HPSE activity was measured using an in-house developed ELISA with a specific anti-HS antibody. B. anti-HSsuggested: NoneNext, plates were washed with PBST and incubated with primary mouse anti-rat IgM HS … SciScore for 10.1101/2020.06.12.20129304: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement ( The local independent ethical committee approved the study protocol (CMO 2020-6344, CMO 2020-6359, CMO 2016-2923). Randomization Samples were randomly distributed over plates. Blinding not detected. Power Analysis not detected. Sex as a biological variable More men than women were included (Table 1). Table 2: Resources
Antibodies Sentences Resources HPSE activity was measured using an in-house developed ELISA with a specific anti-HS antibody. B. anti-HSsuggested: NoneNext, plates were washed with PBST and incubated with primary mouse anti-rat IgM HS antibody JM403 (Amsbio, Abingdon, United Kingdom, cat. no. # 370730-S, RRID: AB_10890960, 1 μg/ml in PBST) for 1 hour at RT. anti-rat IgMdetected: (AMSBIO Cat# 370730-1, RRID:AB_10890960)Subsequently, plates were washed with PBST and incubated with secondary goat anti-mouse IgM HRP antibody (Southern Biotech, Uden, The Netherlands, cat. no. # 1020-05, RRID: AB_2794201, dilution 1:10000 in PBST) for 1 hour a RT. anti-mouse IgMdetected: (SouthernBiotech Cat# 1020-05, RRID:AB_2794201)The plasma samples were 4 times diluted in PBST containing primary mouse anti-rat IgM HS antibody JM403 (1.3 μg/ml) and incubated for 1 hour at RT. anti-rat IgMsuggested: NonePlates were washed with PBST and incubated with secondary goat anti-mouse IgM HRP antibody for 1 hour at RT. anti-mouse IgMsuggested: NoneSoftware and Algorithms Sentences Resources Significance was determined by Fisher’s exact test to compare categorical variables, by Student’s t-test or Mann Whitney test to compare two groups and by Kruskal-Wallis test followed by Dunn’s test to compare more than two groups using GraphPad Prism V.8.4.2 (La Jolla, USA). GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)The graphical abstract was created with BioRender.com. BB and CY share first authorship and AdN and IG are co-second authors, listed in alphabetical order. BioRendersuggested: (Biorender, RRID:SCR_018361)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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SciScore for 10.1101/2020.06.12.20129304: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The local independent ethical committee approved the study protocol (CMO 2020-6344, CMO 2020-6359, CMO 2016-2923).
Consent: All patients admitted to the Radboud university medical center (Radboudumc) with a PCR-proven SARS-CoV-2 infection was asked for informed consent for participation in this study.Randomization Samples were randomly distributed over plates. Blinding not detected. Power Analysis not detected. Sex as a biological variable More men than women were included (Table 1). Table 2: Resources
Antibodies Sentences Resources Next, plates were washed with PBST and incubated with primary mouse anti-rat IgM HS antibody JM403 (Amsbio, Abingdon, United Kingdom, … SciScore for 10.1101/2020.06.12.20129304: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The local independent ethical committee approved the study protocol (CMO 2020-6344, CMO 2020-6359, CMO 2016-2923).
Consent: All patients admitted to the Radboud university medical center (Radboudumc) with a PCR-proven SARS-CoV-2 infection was asked for informed consent for participation in this study.Randomization Samples were randomly distributed over plates. Blinding not detected. Power Analysis not detected. Sex as a biological variable More men than women were included (Table 1). Table 2: Resources
Antibodies Sentences Resources Next, plates were washed with PBST and incubated with primary mouse anti-rat IgM HS antibody JM403 (Amsbio, Abingdon, United Kingdom, cat. no. #370730-S, RRID: AB_10890960, 1 μg/ml in PBST) for 1 hour at RT. anti-rat IgMdetected: (AMSBIO Cat# 370730-1, RRID:AB_10890960)Subsequently, plates were washed with PBST and incubated with secondary goat anti-mouse IgM HRP antibody (Southern Biotech, Uden, The Netherlands, cat. no. #1020-05, RRID: AB_2794201, dilution 1:10000 in PBST) for 1 hour at RT. anti-mouse IgMdetected: (SouthernBiotech Cat# 1020-05, RRID:AB_2794201)The plasma samples were 4 times diluted in PBST containing primary mouse anti-rat IgM HS antibody JM403 (1.3 μg/ml) and incubated for 1 hour at RT. anti-rat IgMsuggested: NonePlates were washed with PBST and incubated with secondary goat anti-mouse IgM HRP antibody for 1 hour at RT. anti-mouse IgMsuggested: NoneSoftware and Algorithms Sentences Resources Significance was determined by Fisher’s exact test to compare categorical variables, by Student’s t-test or Mann Whitney test to compare two groups and by Kruskal-Wallis test followed by Dunn’s test to compare more than two groups using GraphPad Prism V.8.4.2 (La Jolla, USA). GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.06.12.20129304: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement The local independent ethical committee approved the study protocol ( CMO 2020-6344 , CMO 2020-6359 , CMO 2016-2923) . Randomization Samples were randomly distributed over plates . Blinding not detected. Power Analysis not detected. Sex as a biological variable More men than women were included ( Table 1) . Table 2: Resources
Antibodies Sentences Resources Next , plates were washed with PBST and incubated with primary mouse anti-rat IgM HS antibody JM403 ( Amsbio , cat. no. #370730-S , RRID: AB_10890960 , 1 μg/ml in PBST ) for 1 hour at RT . anti-rat IgMsuggested: (AMSBIO Cat# 370730-1, AB_10890960)Sub… SciScore for 10.1101/2020.06.12.20129304: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement The local independent ethical committee approved the study protocol ( CMO 2020-6344 , CMO 2020-6359 , CMO 2016-2923) . Randomization Samples were randomly distributed over plates . Blinding not detected. Power Analysis not detected. Sex as a biological variable More men than women were included ( Table 1) . Table 2: Resources
Antibodies Sentences Resources Next , plates were washed with PBST and incubated with primary mouse anti-rat IgM HS antibody JM403 ( Amsbio , cat. no. #370730-S , RRID: AB_10890960 , 1 μg/ml in PBST ) for 1 hour at RT . anti-rat IgMsuggested: (AMSBIO Cat# 370730-1, AB_10890960)Subsequently , plates were washed with PBST and incubated with secondary goat anti- mouse IgM HRP antibody ( Southern Biotech , cat. no. #1020-05 , RRID: AB_2794201 , dilution 1:10000 in PBST ) for 1 hour at RT . anti- mouse IgM HRPsuggested: (SouthernBiotech Cat# 1020-05, AB_2794201)Software and Algorithms Sentences Resources Significance was determined by Fisher’s exact test to compare categorical variable , by Student’s t-test or Mann Whitney test to compare two groups and by Kruskal-Wallis test followed by Dunn’s test to compare more than two groups using GraphPad Prism V.8.4.2 ( La Jolla , USA) . GraphPad Prismsuggested: (GraphPad Prism, SCR_002798)The graphical abstract was created with BioRender.com. Declaration of Competing Interests The authors have declared that no conflict of interest exists. BioRendersuggested: (Biorender, SCR_018361)Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from OddPub: We did not find a statement about open data. We also did not find a statement about open code. Researchers are encouraged to share open data when possible (see Nature blog).
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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