A Novel In-Cell ELISA Assay Allows Rapid and Automated Quantification of SARS-CoV-2 to Analyze Neutralizing Antibodies and Antiviral Compounds
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SciScore for 10.1101/2020.06.05.135806: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The collection of serum samples has been approved by the ethics committee of the medical faculty of the University of Duisburg-Essen (20-9208-BO). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Anti-SARS-CoV-2 IgG antibodies were detected using ELISA detecting SARS-CoV-2 Spike protein (Euroimmun Medizinische Labordiagnostika, Germany) according to manufacturer’s instructions SARS-CoV-2 icELISA and icNT: Defined doses of SARS-CoV-2 were incubated with different serum dilutions for 1 h at 37°C prior to Vero E6 … SciScore for 10.1101/2020.06.05.135806: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The collection of serum samples has been approved by the ethics committee of the medical faculty of the University of Duisburg-Essen (20-9208-BO). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Anti-SARS-CoV-2 IgG antibodies were detected using ELISA detecting SARS-CoV-2 Spike protein (Euroimmun Medizinische Labordiagnostika, Germany) according to manufacturer’s instructions SARS-CoV-2 icELISA and icNT: Defined doses of SARS-CoV-2 were incubated with different serum dilutions for 1 h at 37°C prior to Vero E6 infection. Anti-SARS-CoV-2 IgGsuggested: NoneThe α-N mAb1 (ABIN6952435), α-N mAb2 (ABIN6952433), α-S Ab (ABIN1031551), and POD-coupled secondary antibodies (Dianova) were used. α-Nsuggested: Noneα-Ssuggested: NonePOD-coupled secondary antibodies (Dianova)suggested: NoneExperimental Models: Cell Lines Sentences Resources Cells, viruses, interferons, and sera: Caco-2 (ATCC HTB-37) and Vero E6 (ATCC CRL-1586) were cultivated in Roswell Park Memorial Institute (RPMI) and Dulbecco’s minimal essential medium (DMEM), respectively, supplemented with 10% (v/v) FCS, penicillin, and streptomycin at 37°C in an atmosphere of 5% CO. Caco-2suggested: NoneSARS-CoV-2 was isolated from a patient sample using Vero E6 and confirmed by SARS-CoV-2 diagnostic qRT-PCR. Vero E6suggested: RRID:CVCL_XD71)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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