Study Importance of Mutation in RAD50 Gene Based on in Silico Analysis of in Vivo Mutation

DNA repair processes occur in all species and the proteins involved are highly conserved. The MRN complex is one of the major sensors of braked DNA and RAD50 as part of the MRN complex supports allosteric connexions over long distances between the zinc hook and the coiled-coil domain. In this study Zebrafish were used as a model to study RAD50 mutation _in vivo_. Knock-out (KO) RAD50 embryos using CRISPR-Cas9 shows embryonic lethality. The final DNA Sanger sequence in each edited sample shows a nucleotide deletion (G) and a nucleotide insertion (A) in the target site, whereupon the aspartic acid residue (D) in the wild type was changed to an asparagine residue (N) in the edited sequences. By _in silico_ analyses of the protein sequence, we found that the nucleotide change could cause a change in the amino acid and protein structure of RAD50 in Zebrafish.