Stability of SARS-CoV-2 RNA in Nonsupplemented Saliva

  1. Isabel M. Ott
  2. Madison S. Strine
  3. Anne E. Watkins
  4. Maikel Boot
  5. Chaney C. Kalinich
  6. Christina A. Harden
  7. Chantal B.F. Vogels
  8. Arnau Casanovas-Massana
  9. Adam J. Moore
  10. M. Catherine Muenker
  11. Maura Nakahata
  12. Maria Tokuyama
  13. Allison Nelson
  14. John Fournier
  15. Santos Bermejo
  16. Melissa Campbell
  17. Rupak Datta
  18. Charles S. Dela Cruz
  19. Shelli F. Farhadian
  20. Albert I. Ko
  21. Akiko Iwasaki
  22. Nathan D. Grubaugh
  23. Craig B. Wilen
  24. Anne L. Wyllie
  25. the Yale IMPACT Research team3

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Abstract

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  1. Version published to 10.3201/eid2704.204199
  2. ScreenIT

    SciScore for 10.1101/2020.08.03.20165233: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: RNA extraction and SARS-CoV-2 detection: RNA was extracted from saliva samples9 collected from COVID-19 inpatients and healthcare workers at the Yale-New Haven Hospital (Yale Human Research Protection Program Institutional Review Boards FWA00002571, Protocol ID.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    Cell culture: Vero-E6 cells (ATCC) were cultured in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (VWR), 1% Penicillin/Streptomycin (Gibco), 100 µg/mL gentamicin (Gibco), and 0.5 µg/mL amphotericin B (Fisher Scientific).
    Vero-E6
    suggested: None
    Software and Algorithms
    SentencesResources
    RNA extraction and SARS-CoV-2 detection: RNA was extracted from saliva samples9 collected from COVID-19 inpatients and healthcare workers at the Yale-New Haven Hospital (Yale Human Research Protection Program Institutional Review Boards FWA00002571, Protocol ID.
    Yale Human Research Protection Program
    suggested: None
    Further statistical analyses were conducted in GraphPad Prism 8.0.0 as described in the text and figure legends.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.08.03.20165233 on medRxiv