IgG Seroconversion and Pathophysiology in Severe Acute Respiratory Syndrome Coronavirus 2 Infection
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SciScore for 10.1101/2020.06.07.20124636: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study is sponsored by St George’s Hospital NHS Trust Foundation and has Institutional Review Board ethical approval (DARTS study - IRAS project ID: 282104; REC reference: 20/SC/0171). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Two-tailed parametric and non-parametric tests were applied as appropriate, with data analysis and display in PRISM (v8). PRISMsuggested: (PRISM, RRID:SCR_005375)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when …
SciScore for 10.1101/2020.06.07.20124636: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study is sponsored by St George’s Hospital NHS Trust Foundation and has Institutional Review Board ethical approval (DARTS study - IRAS project ID: 282104; REC reference: 20/SC/0171). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Two-tailed parametric and non-parametric tests were applied as appropriate, with data analysis and display in PRISM (v8). PRISMsuggested: (PRISM, RRID:SCR_005375)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Limitations of RT-PCR testing include difficulties with sampling, with different sample types and techniques yielding variable results, and diminishing diagnostic yield after peak viral loads that usually occur at the time of symptoms9,15. There may also be biological false positives associated with persistence of viral nucleic acid but no longer infective virus weeks after infection, or in sample handling. Serological testing, and the ability to detect viral antigens, may increase diagnostic accuracy for COVID-19 and our findings support early studies suggesting that these diagnostic modalities should be combined, particularly when RT-PCR results are negative but patients’ symptoms resemble those of COVID-197. Late presentation to secondary care is common and may be part of containment strategies that encourage isolation. One limitation of our study is that it is based mainly on hospitalised patients of whom 1 in 5 did not present with symptoms of COVID-19. Further studies are needed to document antibody dynamics of patients with less severe infections, such as in healthcare workers in eastern France16 and those who may have been missed because they already had low viral loads at the time of consultation. However, our findings will complement the large cross-sectional and longitudinal serological surveys being implemented as high quality tests become more widely available. ELISA NOD values were within a limited dynamic range (dilution studies could not be carried out because...
Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04351646 Not yet recruiting Diagnostics of COVID-19/DARTS (Development and Assessment of… Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a protocol registration statement.
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SciScore for 10.1101/2020.06.07.20124636: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable ( IQR) ! age ! of ! 64 ! ( 52J77) ! years , ! 57 % ! were ! male , ! and ! most ! ( 73 % ) ! had ! one ! or ! more ! coJmorbidities. ! Table 2: Resources
Antibodies Sentences Resources % ! J ! 89 % m ! 149/177) ! had ! already ! seroconverted ! at ! the ! time ! of ! the ! first ! serological ! test . ! Twenty ! six ! percent ! ( 4/15) ! of ! the ! nonJ seroconverters ! were ! followed ! beyond ! 20 ! days , ! suggesting ! that ! 2 ! J ! 8.5 % ! of ! patients ! may ! not ! develop ! detectable ! IgG ! antibody ! responses ! to ! … SciScore for 10.1101/2020.06.07.20124636: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable ( IQR) ! age ! of ! 64 ! ( 52J77) ! years , ! 57 % ! were ! male , ! and ! most ! ( 73 % ) ! had ! one ! or ! more ! coJmorbidities. ! Table 2: Resources
Antibodies Sentences Resources % ! J ! 89 % m ! 149/177) ! had ! already ! seroconverted ! at ! the ! time ! of ! the ! first ! serological ! test . ! Twenty ! six ! percent ! ( 4/15) ! of ! the ! nonJ seroconverters ! were ! followed ! beyond ! 20 ! days , ! suggesting ! that ! 2 ! J ! 8.5 % ! of ! patients ! may ! not ! develop ! detectable ! IgG ! antibody ! responses ! to ! SARSJCoVJ2 ! for ! weeks ! following ! infection . ! ! ! Figures ! 1b ! and ! 1c ! present ! NOD ! IgG ! values ! after ! a ! positive ! IgGsuggested: None<div style="margin-bottom:8px"> <div><b>1b</b></div> <div>suggested: None</div> </div> </td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Our ! study ! highlights ! that ! a ! significant ! proportion ! of ! patients ! with ! COVIDJ19 ! require ! several ! weeks ! postJinfection ! with ! SARSJCoVJ2 ! to ! generate ! antibodies. !</td><td style="min-width:100px;border-bottom:1px solid lightgray"> <div style="margin-bottom:8px"> <div><b>COVIDJ19</b></div> <div>suggested: None</div> </div> </td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">IgG ! antibody ! dynamics ! in ! SARSJCoVJ2 ! infections ! in ! diverse ! populations . ! ! The ! performance ! metrics ! of ! the ! IgG ! ELISA ! used ! here ! ( Fig. ! 1 ! and ! Supplementary ! Table ! 1) ! after ! assessments ! on ! some ! of ! the ! largest !</td><td style="min-width:100px;border-bottom:1px solid lightgray"> <div style="margin-bottom:8px"> <div><b>Fig. ! 1 ! and ! Supplementary ! Table ! 1</b></div> <div>suggested: None</div> </div> </td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">the ! proinflammatory ! cascade ! such ! as ! the ! antiJinterleukinJ 6 ! receptor ! antibody , ! tocilizumab , ! may ! reverse ! disease ! progression ! and ! reduce ! mortality10,11 ! and ! are ! being ! studied ! urgently ! in ! randomised ! clinical ! trials ! ( 20 ! trials ! listed ! currently) . !</td><td style="min-width:100px;border-bottom:1px solid lightgray"> <div style="margin-bottom:8px"> <div><b>antiJinterleukinJ 6 ! receptor !</b></div> <div>suggested: None</div> </div> <div style="margin-bottom:8px"> <div><b>mortality10,11</b></div> <div>suggested: None</div> </div> </td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Another ! likely ! explanation ! is ! that ! relatively ! mild ! infections ! may ! have ! been ! restricted ! to ! the ! mucosal ! cells ! of ! the ! respiratory ! tract , ! where ! antibody ! responses ! are ! dominated ! by ! the ! secretory ! immune ! system ! and ! little , ! if ! any , ! IgG ! antibody ! production ! in ! the ! systemic ! immune ! compartment . ! ! The ! association ! of ! higher !</td><td style="min-width:100px;border-bottom:1px solid lightgray"> <div style="margin-bottom:8px"> <div><b>any , ! IgG</b></div> <div>suggested: None</div> </div> </td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Higher ! antibody ! responses ! are ! also ! seen ! with ! higher ! doses ! of ! nonJreplicating ! Ad5 ! 14 Limitations ! of ! RTJPCR ! testing ! include ! difficulties ! with ! sampling , ! with ! different ! sample ! types ! and ! techniques ! yielding ! variable ! results , ! and ! diminishing ! diagnostic ! yield ! after ! peak ! viral ! loads ! that ! usually ! occur ! at ! the ! time ! of ! symptoms9,15 . !</td><td style="min-width:100px;border-bottom:1px solid lightgray"> <div style="margin-bottom:8px"> <div><b>Ad5</b></div> <div>suggested: None</div> </div> </td></tr></table>Results from OddPub: We did not find a statement about open data. We also did not find a statement about open code. Researchers are encouraged to share open data when possible (see Nature blog).
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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