Culture-Competent SARS-CoV-2 in Nasopharynx of Symptomatic Neonates, Children, and Adolescents

  1. Arnaud G. L’Huillier
  2. Giulia Torriani
  3. Fiona Pigny
  4. Laurent Kaiser
  5. Isabella Eckerle

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Abstract

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  1. Version published to 10.3201/eid2610.202403
  2. ScreenIT

    SciScore for 10.1101/2020.04.27.20076778: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: Clinical data of study patients were retrieved after approval by the institutional review board (Commission Cantonale d’Ethique de la Recherche [CCER] protocol 2020–00835) and documented parental consent in the medical charts.
    Consent: Clinical data of study patients were retrieved after approval by the institutional review board (Commission Cantonale d’Ethique de la Recherche [CCER] protocol 2020–00835) and documented parental consent in the medical charts.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    For assessment of infectious virus, VeroE6 cells were seeded at a density of 8×104 cells/well in a 24 well plate and inoculated with 200 μl of viral transport medium the following day.
    VeroE6
    suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
    A limitation of our study was the small number of children assessed. Furthermore, the use of left-over material received for routine diagnostic purposes could have resulted in suboptimal times between sample collection and storage at −80°C due to transport and diagnostic processing time, resulting in a loss in infectivity and an underestimation of the initial number of viable viral particles. This might have led to a decreased titer of infectious virus and a failure of virus isolation even in the presence of high viral RNA levels. Of note, 2 of 3 samples with a high viral RNA level but unsuccessful virus isolation were collected outside our institution, and thus had longer transport times to the laboratory; the last one was collected in our institution, but the time between specimen collection and processing was 24 hours. The vast majority of patients were managed as outpatients and had to self-isolate at home after diagnosis, so no consecutive sampling was possible to assess infectious virus in multiple samples over the course of disease.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.04.27.20076778v1 on medRxiv