Severe Acute Respiratory Syndrome Coronavirus 2−Specific Antibody Responses in Coronavirus Disease Patients

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1. Version published to 10.3201/eid2607.200841

   Jul 1, 2020
2. 

   ScreenIT

   Jun 5, 2020

   SciScore for 10.1101/2020.03.18.20038059: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After the incubation, the cells were fixed and stained with polyclonal rabbit anti-SARS-CoV antibody (Sino Biological).	anti-SARS-CoV suggested: None
   Antigen-specific antibodies were detected using peroxidase-labeled rabbit anti– human IgG (Dako, https://www.agilent.com) and TMB as a substrate.	Antigen-specific suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Serum Samples: Protein expression: The spike ectodomains of SARS-CoV-2 (residues …

   SciScore for 10.1101/2020.03.18.20038059: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After the incubation, the cells were fixed and stained with polyclonal rabbit anti-SARS-CoV antibody (Sino Biological).	anti-SARS-CoV suggested: None
   Antigen-specific antibodies were detected using peroxidase-labeled rabbit anti– human IgG (Dako, https://www.agilent.com) and TMB as a substrate.	Antigen-specific suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Serum Samples: Protein expression: The spike ectodomains of SARS-CoV-2 (residues 1–1,213; strain Wuhan-Hu-1; GenBank: QHD43416.1), SARS-CoV (residues 1–1,182; strain CUHK-W1; GenBank: AAP13567.1) and MERS-CoV (residues 1-1262; strain EMC; GenBank: YP_009047204.1) were expressed in HEK-293T cells with a C-terminal trimerization motif and Strep-tag using the pCAGGS expression plasmid.	HEK-293T suggested: CCLV Cat# CCLV-RIE 1018, RRID:CVCL_0063)
   Likewise, the SARS-CoV-2 S1 subunit or its subdomains (S;S1, residues 1-682; S1A, residues 1-294; RBD, residues 329-538; GenBank: QHD43416.1) were expressed in 293T cells as described in (Chunyan Wang et al., submitted manuscript;	293T suggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)
   Following incubation, the mixtures were added on Vero-E6 cells and incubated at 37°C for 1 more hour.	Vero-E6 suggested: None
   The PRNT for the German sera was done as described previously using Vero E6 cells (Wölfel et al submitted manuscript, doi:https://doi.org/10.1101/2020.03.05.20030502) (10).	Vero E6 suggested: None
   Software and Algorithms
   Sentences	Resources
   Antigen-specific antibodies were detected using peroxidase-labeled rabbit anti– human IgG (Dako, https://www.agilent.com) and TMB as a substrate.	https://www.agilent.com suggested: (Agilent Technologies, RRID:SCR_013575)
   Statistical Analysis: The correlations between antibody responses detected by different ELISAs were and those detected by PRNT, as the gold standard for CoV serology, were analyzed using GraphPad Prism version 8 (https://www.graphpad.com).	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: Thank you for sharing your data.

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2020.03.18.20038059v1 on medRxiv

   Mar 20, 2020