Little evidence for group differences in 2D:4D ratios based on sexual orientation after adjusting for publication bias

The ratio between the lengths of the second and fourth digits (2D:4D) has been proposed as a putative marker of prenatal androgen exposure and investigated for its potential link to sexual orientation. In a recent updated meta-analysis, Swift-Gallant et al. (2025) reported more male-typical digit ratios in same-sex-oriented women than in heterosexual women, while homosexual men showed more female-typical ratios than heterosexual men. Their analyses found no evidence of publication bias for female sexual orientation but did for male sexual orientation, with effects remaining statistically significant after adjustment. Here, we reanalyzed the dataset compiled by Swift-Gallant et al. (2025) using Robust Bayesian Meta-Analysis (RoBMA), a framework that models uncertainty across multiple sources, including the presence or absence of an effect, heterogeneity, and publication bias. Our analyses showed little evidence for group differences in 2D:4D ratios by sexual orientation: RoBMA indicated moderate evidence against differences between homosexual and heterosexual women, with moderate to strong evidence of publication bias, and for men, moderate evidence against a right-hand difference but a small left-hand effect moderated by publication status. Thus, when publication bias was modeled directly using Bayesian model averaging of complementary bias adjustments, the evidence for a difference in 2D:4D ratios according to sexual orientation was substantially weakened, with this evidence for publication bias not attributable to unmodeled heterogeneity. Overall, these findings challenge the view that the 2D:4D ratios provides a reliable marker of prenatal androgen exposure underlying variation in sexual orientation and question assertions that publication bias in this literature is negligible or reversed.