The Use of Saliva as a Diagnostic Specimen for SARS CoV-2 Molecular Diagnostic Testing for Pediatric Patients
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SciScore for 10.1101/2020.11.11.20223800: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: To determine if saliva was a suitable specimen to use to test children for the presence of the SARS-CoV-2 virus, institutional review board (IRB) approval was obtained and a two-part study was undertaken.
Consent: After informed consent was obtained, subjects were asked to not to eat or drink for 30 minutes prior to saliva collection and then each subject provided a saliva specimen.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share …
SciScore for 10.1101/2020.11.11.20223800: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: To determine if saliva was a suitable specimen to use to test children for the presence of the SARS-CoV-2 virus, institutional review board (IRB) approval was obtained and a two-part study was undertaken.
Consent: After informed consent was obtained, subjects were asked to not to eat or drink for 30 minutes prior to saliva collection and then each subject provided a saliva specimen.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Our study has some limitations. Although over 500 patients gave a saliva specimens as part of this study, the number with a positive PCR test result was low, limiting our number of contemporaneous positive NP and saliva specimens. However, we overcame this by using saliva spiked with positive NP specimens and comparing the analytical performance using CT values as a more precise measure. Other authors have found comparable results using the CDC’s version of the SARS-CoV-2 using unprocessed saliva in symptomatic adult individuals.10 Chong et al. found testing of saliva collected from children to be less sensitive than testing NP specimens in viral transport medium. We initially obtained similar results when testing unprocessed saliva, however, when we collected saliva from children with the SpectrumDNA saliva collection kit, we found comparable sensitivity as measured by CT values from NP and saliva specimens. In conclusion, our study findings indicate that saliva collected from COVID-19 infected symptomatic and asymptomatic children that is mixed with SpectrumDNA saliva stabilizing solution yields clinically comparable PCR results compared to NP specimens. Use of saliva SARS-CoV-2 testing will enable greater access to sensitive PCR testing and may be advantageous for “back to school” testing programs for children.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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