Prior infection by seasonal coronaviruses, as assessed by serology, does not prevent SARS-CoV-2 infection and disease in children, France, April to June 2020

Article activity feed

1. Version published to 10.2807/1560-7917.es.2021.26.13.2001782

   Apr 1, 2021
2. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2020.06.29.20142596: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   In a fraction of samples, we assessed by LIPS tests, antibodies to the nucleoprotein and to the full spike ectodomain in a pre-fusion conformation for SARS-CoV-2, and the four human coronaviruses (HKU1, NL63, OC43, and 229E).	SARS-CoV-2 suggested: None HKU1 suggested: None NL63 suggested: None
   Software and Algorithms
   Sentences	Resources
   We also included in this study during the same period patients presenting with a MIS disease, as defined by the American Heart Association21.	American Heart suggested: …

   SciScore for 10.1101/2020.06.29.20142596: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   In a fraction of samples, we assessed by LIPS tests, antibodies to the nucleoprotein and to the full spike ectodomain in a pre-fusion conformation for SARS-CoV-2, and the four human coronaviruses (HKU1, NL63, OC43, and 229E).	SARS-CoV-2 suggested: None HKU1 suggested: None NL63 suggested: None
   Software and Algorithms
   Sentences	Resources
   We also included in this study during the same period patients presenting with a MIS disease, as defined by the American Heart Association21.	American Heart suggested: (American Heart Association, RRID:SCR_007210)
   Statistical analyses were conducted with Excel or GraphPad Prism 8 (GraphPad Software, LLC).	Excel suggested: None GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)
   Data were processed with R 3.6.3 using GGPlot2 with GGally for matrices of plots, and ggfortify for PCA plots packages.	GGPlot2 suggested: (ggplot2, RRID:SCR_014601)

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:

   However, this represents a limitation as cases of infection have been reported to be seronegative as revealed by isolated T-cell responses31. Seasonal HCoVs include alphacoronaviruses (229E and NL63) and betacoronaviruses lineage A (OC43 and HKU1), which primarily replicate in the respiratory tract and mostly cause common colds32. These viruses show a worldwide distribution and multiple HCoV infections in various combinations are common10,32–34,35. Infection takes place in very early childhood and seroprevalence studies show very high prevalence rates36, up to 100% in adult populations10,37,38. It is therefore very likely that infection by HCoVs preceded infection by SARS-CoV-2 in our cohort. Previous seasonal HCoV infections could have an impact on SARS-CoV-2 replication. Indeed, antibodies are unlikely to act as primary effectors of protection, as there is no or very low11 cross neutralization between these coronaviruses, but antibodies serve as an indicator of underlying cellular responses. We found no evidence of cross-protective immunity linked to previous infection by seasonal HCoVs. First, similar seasonal HcoV prevalence was found in SARS-CoV-2 positive versus negative patients. Second, on a quantitative side, there was no significant correlation between SARS-CoV-2 and any HCoV antibody titres, whatever the antigen considered (S or N). On the contrary, the level of SARS-CoV-2 antibodies to N and S were correlated, which corresponds to a good internal control. This was...

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a protocol registration statement.

   ---

   Read the original source
3. 

   ScreenIT

   Jul 2, 2020

   SciScore for 10.1101/2020.06.29.20142596: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement

   not detected.

   Randomization

   not detected.

   Blinding

   not detected.

   Power Analysis

   not detected.

   Sex as a biological variable

   not detected.

   Cell Line Authentication

   not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Antibody frequency and titres against Nucleocapsid (N) and Spike (S) of the four seasonal coronaviruses (NL63, HKU1, 229E, OC43) were measured in a subset of seropositive patients (54 SARS-CoV-2 (HOS-P subgroup) and 15 MIS (MIS-P subgroup)), and in 118 matched SARS-CoV-2 seronegative patients (CTL subgroup).	NL63 suggested: None
   The level of anti-SARS-CoV-2 antibodies was not …

   SciScore for 10.1101/2020.06.29.20142596: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement

   not detected.

   Randomization

   not detected.

   Blinding

   not detected.

   Power Analysis

   not detected.

   Sex as a biological variable

   not detected.

   Cell Line Authentication

   not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Antibody frequency and titres against Nucleocapsid (N) and Spike (S) of the four seasonal coronaviruses (NL63, HKU1, 229E, OC43) were measured in a subset of seropositive patients (54 SARS-CoV-2 (HOS-P subgroup) and 15 MIS (MIS-P subgroup)), and in 118 matched SARS-CoV-2 seronegative patients (CTL subgroup).	NL63 suggested: None
   The level of anti-SARS-CoV-2 antibodies was not significantly different in children who had prior seasonal coronavirus infection.	anti-SARS-CoV-2 suggested: None
   In a fraction of samples, we assessed by LIPS tests, antibodies to the nucleoprotein and to the full spike ectodomain in a pre-fusion conformation for SARS-CoV-2, and the four human coronaviruses (HKU1, NL63, OC43, and 229E).	HKU1 suggested: None
   2-Profiling SARS-CoV-2 antibody responses in HOS-P and MIS-P patients HOS-P patients Ab profile was characterized by a dominant S2 response compared to responses to S1, N and to the full S ectodomain (Figure 3).	2-Profiling SARS-CoV-2 suggested: None
   Neutralizing titres of PNT+ and PRNT+ sera of MIS-P patients were similar to those of HOS-P patients (Figure 3). 4-Relationship between SARS-CoV-2 and seasonal HCoV infections We compared the prevalence of anti-N and -S antibodies against the four seasonal HCoVs in a subpopulation of children among the HOS-P (n=54), MIS-P (n=15) and CTL (n=118) groups (Figure 1).	anti-N suggested: (Novus Cat# NB500-118, AB_10001231)
   This would diminish or impair SARS-CoV-2 antibody responses, which should therefore be lower in patients with high anti-HCoV Ab levels.	anti-HCoV suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Although those Abs neutralized entry of SARS-CoV-2 S-pseudo-typed lentiviruses in HEK-293T cells mediated by the spike protein, the clinical relevance of the SARS-CoV-2 pseudo-neutralisation test is questionable, because the mechanism of entry did not involve the ACE2 receptor of the virus.	HEK-293T suggested: KCB Cat# KCB 200744YJ, CVCL_0063
   Software and Algorithms
   Sentences	Resources
   We searched in PubMed, MedRxiv and BioRxiv for publications from inception to June 15, 2020, using the terms “COVID-19, SARS-CoV-2, children, serology, Kawasaki, Corona Virus”.	PubMed suggested: (PubMed, SCR_004846) <div style="margin-bottom:8px"> <div><b>BioRxiv</b></div> <div>suggested: (bioRxiv, <a href="https://scicrunch.org/resources/Any/search?q=SCR_003933">SCR_003933</a>)</div> </div> </td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">We also included in this study during the same period patients presenting with a MIS disease, as defined by the American Heart Association21.</td><td style="min-width:100px;border-bottom:1px solid lightgray"> <div style="margin-bottom:8px"> <div><b>American Heart</b></div> <div>suggested: (American Heart Association, <a href="https://scicrunch.org/resources/Any/search?q=SCR_007210">SCR_007210</a>)</div> </div> </td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Statistical analyses were conducted with Excel or GraphPad Prism 8 (GraphPad Software, LLC).</td><td style="min-width:100px;border-bottom:1px solid lightgray"> <div style="margin-bottom:8px"> <div><b>Excel</b></div> <div>suggested: None</div> </div> <div style="margin-bottom:8px"> <div><b>GraphPad Prism</b></div> <div>suggested: (GraphPad Prism, <a href="https://scicrunch.org/resources/Any/search?q=SCR_002798">SCR_002798</a>)</div> </div> <div style="margin-bottom:8px"> <div><b>GraphPad</b></div> <div>suggested: (GraphPad Prism, <a href="https://scicrunch.org/resources/Any/search?q=SCR_002798">SCR_002798</a>)</div> </div> </td></tr><tr><td style="min-width:100px;vertical-align:top;border-bottom:1px solid lightgray">Data were processed with R 3.6.3 using GGPlot2 with GGally for matrices of plots, and ggfortify for PCA plots packages.</td><td style="min-width:100px;border-bottom:1px solid lightgray"> <div style="margin-bottom:8px"> <div><b>GGPlot2</b></div> <div>suggested: (ggplot2, <a href="https://scicrunch.org/resources/Any/search?q=SCR_014601">SCR_014601</a>)</div> </div> </td></tr></table> Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations. Results from OddPub: We did not find a statement about open data. We also did not find a statement about open code. Researchers are encouraged to share open data when possible (see Nature blog). About SciScore SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.

   Read the original source
4. 

   Version published to 10.1101/2020.06.29.20142596v1 on medRxiv

   Jun 30, 2020