Elevated Antibody Titers in Abdala Vaccinees Evaluated by Elecsys® Anti-SARS-Cov-2 S Highly Correlate with UMELISA SARS-Cov-2 ANTI RBD, ACE-2 Binding Inhibition and Viral Neutralization Assays

  1. Gilda Lemos-Perez
  2. Sheila Chavez-Valdes
  3. Hany Gonzalez-Formental
  4. Giselle Freyre-Corrales
  5. Amalia Vazquez-Arteaga
  6. Beatriz Alvarez-Acevedo
  7. Lismary Avila-Díaz
  8. Ricardo U Martínez-Rosales
  9. Yahima Chacon-Quintero
  10. Edelgis Coizeau-Rodriguez
  11. Ariel Palenzuela-Diaz
  12. Enrique Noa-Romero
  13. Gerardo E Guillen-Nieto

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Abstract

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  1. Version published to 10.26502/jbb.2642-91280055
  2. ScreenIT

    SciScore for 10.1101/2021.10.18.21265169: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsConsent: Ethics: Subjects gave their consent after being informed that the immediate use of the samples was to determine post vaccination antibody titers and there were possibilities of their use in other related research.
    IRB: The study was approved by CIGB Ethics Committee in Havana, Cuba.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    UMELISA SARS-CoV-2 ANTI RBD assay: Class IgG antibodies were quantified by UMELISA SARS-CoV-2 ANTI RBD, approved by CECMED (Cuban Center for State Control of Medicines and Medical Devices) and commercialized by the ImmunoAssay Center (Cuba) [27].
    Class IgG
    suggested: None
    , biotin-bound anti-human IgG monoclonal antibody is then added, which will bind to the antibodies fixed in the previous step.
    anti-human IgG
    suggested: None
    Antibody neutralization properties were determined using an “in-house” surrogate virus neutralization test (sVNT) measuring RBD-ACE2 (angiotensin-converting enzyme 2) inhibition (CIGB, Cuba) and confirmed by a live viral neutralization test (Civilian Defense Scientific Research Center, Cuba).
    angiotensin-converting enzyme 2
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Vero E6 cells are incubated in absence or presence of diluted sera and overlaid with viral suspension.
    Vero E6
    suggested: RRID:CVCL_XD71)
    Software and Algorithms
    SentencesResources
    Inhibition values were plotted in GraphPad Prism 8.0.2 and the neutralization titer was defined as the concentration that showed 50% inhibition (inhibitory concentration, IC50) as determined by log (inhibitor) vs. normalized response – variable slope model.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.10.18.21265169 on medRxiv