A Drug Candidate for Treating Adverse Reactions Caused by Pathogenic Antibodies Inducible by SARS-CoV-2 Virus and Vaccines
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
-
SciScore for 10.1101/2021.07.13.452194: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: The protocol for the animal experiment was approved by the Laboratory Animal Welfare and Ethics Committee of Hangzhou Normal University. Sex as a biological variable Animal: SPF-grade C57BL/6J pregnant mice at pregnancy (embryonic) day E13-E14 were purchased from Shanghai SLAC Laboratory Animal Co. Randomization The animals were randomly divided into groups as needed, two pregnant mice for each group at every experiment. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Production of human anti-SARC-CoV-2 spike monoclonal antibodies: Previously reported naturally occurring human monoclonal antibodies of B38 … SciScore for 10.1101/2021.07.13.452194: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: The protocol for the animal experiment was approved by the Laboratory Animal Welfare and Ethics Committee of Hangzhou Normal University. Sex as a biological variable Animal: SPF-grade C57BL/6J pregnant mice at pregnancy (embryonic) day E13-E14 were purchased from Shanghai SLAC Laboratory Animal Co. Randomization The animals were randomly divided into groups as needed, two pregnant mice for each group at every experiment. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Production of human anti-SARC-CoV-2 spike monoclonal antibodies: Previously reported naturally occurring human monoclonal antibodies of B38 (Wu et al., 2020), REGN10987 (Hansen et al., 2020), and CR3022-B6 (Rouet et al., 2020), specific to the receptor binding domain (RBD) of the spike protein one (S1) of the SARS-CoV-2 virus were re-produced as previously reported (HuaAn McAb Biotechnology). anti-SARC-CoV-2suggested: NoneCR3022-B6suggested: NoneAntibody injection: The purified and endotoxin-free IgG of the anti-coronavirus antibodies used for the animal model include rabbit polyclonal anti-COVID-19 S1, anti-SARS-CoV S, and the human monoclonal anti-COVID-19 S1, B38 (Wu et al., 2020), REGN10987 (Hansen et al., 2020), and CR3022-B6 (Rouet et al., 2020). anti-coronavirussuggested: Noneanti-SARS-CoV S,suggested: (BioLegend Cat# 944301, RRID:AB_2890871)In addition, two dosages of BH-103 injection (IP) at either day before or the same day of each antibody injection of the 4 pathogenic antibodies (anti-COVID-19 S1, anti-SARS-CoV S, REGN10987, and B38) were administrated, respectively. anti-COVID-19suggested: Noneanti-SARS-CoV Ssuggested: (BioLegend Cat# 944301, RRID:AB_2890871)REGN10987suggested: NoneExperimental Models: Cell Lines Sentences Resources Digestion of A549 and NB4 cells with neuraminidase: The cells of human lung epithelium cell line A549 (ATCC), and human acute promyelocytic leukemia cell line NB4 (ATCC) were washed once with the digestion buffer for neuraminidase (Roche), the cells were centrifuged at 2000 rpm for 5 minutes, and the supernatant was discarded. A549suggested: NoneA549 or NB4 cells without the treatment of neuraminidase were used as controls. NB4suggested: CLS Cat# 300299/p11500_NB-4, RRID:CVCL_0005)Experimental Models: Organisms/Strains Sentences Resources Animal: SPF-grade C57BL/6J pregnant mice at pregnancy (embryonic) day E13-E14 were purchased from Shanghai SLAC Laboratory Animal Co. C57BL/6Jsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-