AI334 and AQ806 antibodies recognize the spike S protein from SARS-CoV-2 by ELISA

  1. Philippe Hammel
  2. Anna Marchetti
  3. Wanessa C Lima
  4. Kelvin Lau
  5. Florence Pojer
  6. David Hacker
  7. Pierre Cosson

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Abstract

We tested 10 recombinant antibodies directed against the spike S protein from SARS-CoV-1. Among them, antibodies AI334 and AQ806 detect by ELISA the spike S protein from SARS-CoV-2.

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  1. ScreenIT

    SciScore for 10.1101/2020.05.08.084103: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Antibodies: ABCD_AA831, ABCD_AF167, ABCD_AF618, ABCD_AH286, ABCD_AH287, ABCD_AH971, ABCD_AH974, ABCD_AI334, ABCD_AQ601, ABCD_AQ602, and ABCD_AQ806 antibodies (ABCD nomenclature, https://web.expasy.org/abcd/) were produced by the Geneva Antibody Facility (http://www.unige.ch/medecine/antibodies/) as mini-antibodies with the antigen-binding scFv portion fused to a mouse IgG2A Fc.
    ABCD_AQ806
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    HEK293 suspension cells (growing in FreeStyle™ 293 Expression Medium, Gibco #12338) were transiently transfected with the vector coding for the scFv-Fc of each antibody.
    HEK293
    suggested: None
    Software and Algorithms
    SentencesResources
    Antibodies: ABCD_AA831, ABCD_AF167, ABCD_AF618, ABCD_AH286, ABCD_AH287, ABCD_AH971, ABCD_AH974, ABCD_AI334, ABCD_AQ601, ABCD_AQ602, and ABCD_AQ806 antibodies (ABCD nomenclature, https://web.expasy.org/abcd/) were produced by the Geneva Antibody Facility (http://www.unige.ch/medecine/antibodies/) as mini-antibodies with the antigen-binding scFv portion fused to a mouse IgG2A Fc.
    https://web.expasy.org/abcd/
    suggested: (ExPASy ABCD database, RRID:SCR_017401)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • No funding statement was detected.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  2. Version published to 10.24450/journals/abrep.2020.e186
  3. Version published to 10.1101/2020.05.08.084103v1 on bioRxiv