Cytotoxic T Lymphocytes Targeting a Conserved SARS-CoV-2 Spike Epitope are Efficient Serial Killers
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SciScore for 10.1101/2022.01.24.477535: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication Contamination: A375 cells were confirmed for absence of mycoplasma contamination using real-time PCR. Table 2: Resources
Antibodies Sentences Resources We used AF647-Annexin V (ThermoFisher, Waltham, Mass, USA, Cat #A23204) as a death marker and anti-CD28 antibody (ThermoFisher, Waltham, Mass, USA, Cat #16-0289-81, Clone CD28.2) to stimulate the secretion of IFNγ. anti-CD28suggested: (Thermo Fisher Scientific Cat# 16-0289-81, RRID:AB_468926)Experimental Models: Cell Lines Sentences Resources We used PKH26 and PKH67 dye (Sigma-Aldrich, Saint Louis, MO, … SciScore for 10.1101/2022.01.24.477535: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication Contamination: A375 cells were confirmed for absence of mycoplasma contamination using real-time PCR. Table 2: Resources
Antibodies Sentences Resources We used AF647-Annexin V (ThermoFisher, Waltham, Mass, USA, Cat #A23204) as a death marker and anti-CD28 antibody (ThermoFisher, Waltham, Mass, USA, Cat #16-0289-81, Clone CD28.2) to stimulate the secretion of IFNγ. anti-CD28suggested: (Thermo Fisher Scientific Cat# 16-0289-81, RRID:AB_468926)Experimental Models: Cell Lines Sentences Resources We used PKH26 and PKH67 dye (Sigma-Aldrich, Saint Louis, MO, USA, Cat #PKH26GL and #PKH67GL) for A375 and T cells staining, accordingly. A375suggested: CLS Cat# 300110/p852_A-375, RRID:CVCL_0132)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 18. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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