A Comparison of DNA/RNA Extraction Protocols for High-Throughput Sequencing of Microbial Communities
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2020.11.13.370387: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Human urine samples included samples from female and male individuals. Table 2: Resources
Software and Algorithms Sentences Resources For shallow shotgun metagenomics data, raw sequence files were demultiplexed using BaseSpace (Illumina, La Jolla, CA), quality-filtered using Atropos [34] and human read depleted by alignment to human reference genome GRCh38 using bowtie2 [35]. BaseSpacesuggested: (BaseSpace, RRID:SCR_011881)bowtie2suggested: (Bowtie 2, RRID:SCR_016368)Results from OddPub: Thank you for sharing your code and data.
Results from LimitationRecog…SciScore for 10.1101/2020.11.13.370387: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Human urine samples included samples from female and male individuals. Table 2: Resources
Software and Algorithms Sentences Resources For shallow shotgun metagenomics data, raw sequence files were demultiplexed using BaseSpace (Illumina, La Jolla, CA), quality-filtered using Atropos [34] and human read depleted by alignment to human reference genome GRCh38 using bowtie2 [35]. BaseSpacesuggested: (BaseSpace, RRID:SCR_011881)bowtie2suggested: (Bowtie 2, RRID:SCR_016368)Results from OddPub: Thank you for sharing your code and data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
