Differential Effects of Cultural Performing Arts on Anti-Aging Biomarkers and Well-Being: A Non- Randomized Pre–Post Comparative Study of Traditional Noh/Kyogen and Comedy

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Abstract

This study examined how cultural performing arts affect psychological well-being and stress-related biomarkers, comparing traditional Japanese Noh and Kyogen (TNKP) with live comedy (CLP). Participants aged 18–64 years were assessed before and after attending performances (TNKP: n = 41; CLP: n = 119). Salivary biomarkers (cortisol, dehydroepiandrosterone [DHEA], and their ratio), psychological outcomes (State-Trait Anxiety Inventory), and well-being (PERMA framework) were measured pre- and post-show. Laughter frequency during performance was higher in the CLP than in the TNKP. Cortisol levels decreased significantly in both groups. However, a significant decrease in the cortisol/DHEA (C/D) ratio was observed only in the TNKP, driven by cortisol reduction while DHEA remained stable. State anxiety decreased significantly in the CLP. Both groups showed significant improvements in positive emotion, accomplishment, and negative emotion. The CLP also showed significant improvements in overall well-being, meaning, relationship, engagement, and loneliness, with no significant changes in these domains in the TNKP. Both performances reduced stress and improved some aspects of well-being. Comedy led to faster, broader psychological gains. Noh and Kyogen improved neuroendocrine balance, suggesting anti-aging benefits. Each art form offered distinct health effects.

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