A green digestion method for biological samples with hydrogen peroxide as the main digestion solvent
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The present study examines an environmentally friendly, efficient and safe digestion method for biological samples using hydrogen peroxide (H 2 O 2 ) as the primary digestant, aiming to replace the widely used traditional digestion methods based on concentrated acids such as nitric acid (HNO 3 ), hydrochloric acid, and sulphuric acid (H 2 SO 4 ). Initially, the concentrations of H 2 O 2 and HNO 3 were optimised, followed by adjusting factors such as pressure, temperature and time to establish a heating programme that ensures uniform and complete digestion of various representative animal and plant tissues under mild conditions. Under low-pressure conditions, a digestion solution containing 8%–16% H 2 O 2 and 3%–4% HNO 3 effectively digested various biological materials, yielding excellent outcomes. Based on a comprehensive ecological scale analysis, this method exhibits advantages in terms of environmental friendliness, safety, cost-effectiveness and high efficiency, ultimately achieving a total score of 85, fully validating its potential and value as an outstanding green digestion method.
