Chitosan-collagen scaffolds cross-linked with genipin demonstrated cytocompatibility at different levels of bioanalysis with human adipose-derived mesenchymal stem cells for regenerative medicine: a research article

Introduction: One in five people over 65 years of age benefiting from in vitro-generated tissues and organs. This study aimed to analyze the cytocompatibility of Adipose-Derived Stem Cells (ADSC) at the chromosomal/chromatid level in the presence of the chitosan-collagen-genipin biomatrix. Methods: ADSC was obtained from human lipoaspirate by enzymatic method and centrifugation and then cultured in Petakas (control) and the chitosan-collagen-genipin biomatrices, with and without cryopreservation. ADSC were characterized for adhesion and proliferation by conventional optical microscopy, phase contrast and scanning electron microscopy, immunophenotyping, cell viability with Trypan blue test, chromosomal and chromatid stability by traditional cytogenetic techniques, neoplastic potential by Papanicolau test and cell differentiation capacity in three types of tissues. Results: The biomatrix intercrossed with genipin 0.75% v/v was selected for studies in this article since it presented good physicochemical stability. After cultivation in Petakas and biomatrices, ADSC maintained their intrinsic functions of adhesion, proliferation, and differentiation. Cytogenetic tests of ADSC after different culture conditions in biomatrices revealed no statistically significant differences (p<0.05) to control cells (cultured in Petakas). Discussion: Biomatrix intercrossed with genipin 0.75% v/v can stimulate the expression of ADSC activities. The presence of cations and anions on individual polymer chains can stimulate the cellular activities of ADSC without causing cellular stress or loss of quiescence in these cells. Conclusion: The chitosan-collagen-genipin biomatrix with genipin at 0.75% v/v was shown to be biocompatible both at the cellular level and at the chromosomal/chromatid level of adipose mesenchymal stem cells.