Discovery of a novel adult-plant resistance locus conferring broad-spectrum stripe rust immunity in spelt wheat and its suppression in bread wheat
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Adult-plant resistance (APR) genes provide durable, non-race-specific resistance against wheat rust diseases. We identified a novel APR locus in the spelt wheat cultivar CDC Silex that confers non-race-specific near-immune adult-plant resistance to stripe rust. The trait segregates as a single dominant gene and bulked segregant analysis sequencing (BSA-seq) mapped the locus, QYr.cbl-5D , to a single 0.31 Mb interval on chromosome 5D, confirming monogenic inheritance. Interestingly, introgression into bread wheat suppresses this resistance, suggesting the presence of a dominant suppressor factor in the bread wheat genome. We generated a scaffold-scale assembly of CDC Silex to annotate the locus and its flanking region on 5D. Within a ± 1 Mb interval flanking the QYr.cbl-5D peak, six genes were identified, of which five had no predicted function and one encodes a C2H2 zinc-finger transcription factor. Future work will aim to clone this APR and its suppressor in bread wheat. Flanking and linked SNPs from this region will enable marker-assisted selection to deploy this APR locus, providing a durable resistance resource to support sustainable wheat breeding and reduce reliance on fungicides.
