Establishment of an Efficient Tissue Culture and Regeneration System for Lycium chinense var. macrophyllum and Its Callus Culture Characteristics

Lycium chinense var. macrophyllum , a unique Chinese medicinal and edible plant with high nutritional value and broad application prospects, is limited by inefficient traditional breeding methods. Taking different tissur as explants, this study explored 6-BA and NAA ratio effects on tissue culture and optimized disinfection. The results showed that the optimal disinfection procedure was rinsing with running water for 2 h soaking in 75% ethanol for 60 s, after rinsing with sterile water, sterilizing with 1% sodium hypochlorite for 20 min. The optimal media for callus induction, bud differentiation and root induction were MS + 6-BA 0.3 mg/L + NAA 1.0 mg/L, MS + 6-BA 0.3 mg/L + NAA 0.5 mg/L, and MS + NAA 0.2 mg/L, respectively. After acclimatization, the survival rate of transplanted regenerated plants reached 95%. This study established an efficient tissue culture and regeneration system, providing technical support for its germplasm preservation and genetic improvement.