Characterization, ScoT based diversity analysis and molecular identification of a novel rhizobacterial (Streptococcus thermophilus) isolates from dhaman (Cenchrus setigerus) in arid zone of Rajasthan

Novel solutions for plant growth enhancements are required to ease the burden imposed on our environment and other resources. Here we look at potential solutions to these issues by examining some of the research conducted regarding the biological applications of plant growth promoting rhizobacteria (PGPR) associated in Dhaman grass (Cenchrus setigerus). There is a lack of information regarding the rhizospheric microbial communities of Cenchrus setigerus, which can play a vital role in promoting plant root growth. The current research aimed at discovering plant growthpromoting rhizobacteria (PGPR) in the rhizosphere of soil samples collected from around the root zone of Cenchrus setigerus growing Jodhpur; Jaisalmer and Bikaner districts of Rajasthan, without causing harm and their relevance in desert grass has grown due to nutritive used in feed and fodder for arid environmental conditions. The isolated bacterial strains were characterized based on different morphological, biochemical, and molecular markers, along with soil attributes such as nutrient status and soil microbiological activities. Among the isolates, eighteen produced indole acetic acid (IAA), ten solubilized phosphate, and fourteen showed hydrogen cyanide (HCN) production. In this study, the Start Codon Targeted (SCoT) marker system was found to be best effective technique to estimating valuable in the genetic diversity studies and detecting 100% polymorphism information content (PIC) among the bacterial communities. Population structure analysis (based on ΔK values) indicated the presence of genetically pure bacterial isolates. Based on molecular identification validation of sequences from selected pure isolates uncovered four that exhibited high similarity to Streptococcus strains found in public genomic databases, as established through partial 16S rRNA-ITS gene sequencing, these isolates were further analyzed for bioinformatics study sequences have been submitted to NCBI with accession numbers PV566939, PV566921, PV566757, and PV566932, and were classified as Streptococcus thermophilus. The findings of the present investigation showed that thermophilles bacteria isolated from the rhizosphere of Dahman grass in the arid zone of Jodhpur, Rajasthan, possess promising plant growth promoting characteristics. The discovery of these novel PGPR strains could probably assist Cenchrus setigerus in improving their mechanisms of adaptation to drought stress and may be potential application as biofertilizer, and biodecomposers, and in the future, as biocontrol agents for crop improvement under sustainable agriculture in arid regions.