Synthesis, characterization, and molecular docking analysis of a novel nanocarrier for gene therapy in renal cell carcinoma

Today, nanotechnology has emerged as a promising approach in biomedical fields such as diagnosis and treatment of disease. Gene therapy is a technique that can treat a deficiency by sending a gene into the targeted cell instead of using drugs and surgery and can cause the least harm to humans. The combination of gene therapy techniques and nanotechnology opens a new way to improve clinical outcomes. The present study aimed to develop a high-potential carrier for gene delivery by synthesizing Eggshell/Citric Acid/Chitosan nanocomposites using the co-precipitation method. The FTIR spectrum confirmed the chemical structure of the prepared nanocomposite. TEM analysis revealed plate-like structures with an average diameter of 300–400 nm and DLS analysis showed the average hydrodynamic size of the synthesized product in the range of 700–4000 nm. In silico molecular docking using the MVD method in Schrodinger software was done and predicted favorable interactions between synthesized nanocomposite and enzyme F218V AtRCCR with − 137.39 KJmol ^− 1 for inhibition the active site of F218V AtRCCR, serving this nanocomposite as an appropriate carrier for gene delivery and potential product for in vivo studies to renal cell carcinoma treatment.