Biogenic Synthesis of Intracellular Silver Nanoparticle by Endophytic Ascomycetes Fungi From Calotropis gigantea Seeds (L.) R. Br

The biosynthesis of monodispersed nanoparticles, along with the determination of potentially responsible biomolecules, is the major bottleneck in the area of bio-nanotechnology research. The current investigation focused to synthesize the Silver Nanoparticles (AgNPs) using an endophytic fungus isolated from Calotropis gigantea (L. Br) seed, located in Chennai, India. The fungus was identified for its morphological and molecular characteristics. The fungus identified as Pichia kudriavzevii strain based on 16SrRNA sequencing. The produced particles were spherical shaped in nature and shows the surface plasma resonances 10nm. FTIR spectra of nanoparticle showed the C = C bonding, N-compound, C-O stretching vibration denotes the presences of alkene, protein and aromatic compound on the surface of silver nanoparticle produced by Pichia kudriavzevii . Synthesised NanoParticle revealed thee mean negative zeta potential and electrophoretic mobility of -29.8 mV and − 0.000255 cm ² /Vs respectively. The nanoparticles produced were proteinaceous compounds as capping agents with − 29.8mV zeta potential and revealed the antibacterial activity against gram positive bacterial strain.