Characterization of Crude Cellulase Produced by Bacillus licheniformis OE-1 from the Diyadin Hot Springs, Ağrı Province

In this study, we characterized the crude cellulase produced by Bacillus licheniformis OE-1, a strain isolated from the thermal springs of Diyadin District (Ağrı Province, Turkey) and examined the effects of various compounds on its activity. The enzyme exhibited an optimum activity at 60 °C and pH 9.0. Using carboxymethyl cellulose (CMC) as the substrate, the enzyme displayed a Vmax of 2.33 U mL⁻¹ and a Km of 0.0348 mM. When the effects of EDTA, KCl, MgSO ₄ , KI, and SDS compounds on the cellulase enzyme were investigated, it was observed that these compounds inhibit the enzyme. By plotting a Lineweaver-Burk graph, the IC ₅₀ values of these compounds were calculated, and the inhibition types were determined.