Effects of Carboplatin and Cisplatin on C33a Cell Line

Platinum-based chemotherapeutic drugs such as Carboplatin and Cisplatin are widely used in cancer treatments due to their cytotoxic effects on Cancer cells. This Paper mainly focuses on the study of the effect of drugs on a C33A cell line in the commonly used Cervical cancer model. This study employs two independent methodologies namely MTT Assay for determining cell viability and Molecular Docking targeting Human Peroxiredoxin 2 to predict the predominant binding affinity. This study aims to provide insights into the drug's efficacy and potential differences in their impact on the cancer cell line. Moreover, the Molecular Docking approach is employed to investigate the interaction between carboplatin and cisplatin with human peroxiredoxin 2 (Prx2) a protein involved in redox regulation and implicated in cancer progression. The study’s Molecular docking aspects focus on the interaction between the two drugs with Human Prx2. Peroxiredoxin 2 is chosen as the docking target due to its significance in redox homeostasis and may play a role in the way cells react to drugs based on platinum, it was selected as the Docking target. A thorough understanding of the cellular response to carboplatin and cisplatin is made possible by the integration of the MTT assay result with Molecular docking data which also brings information on possible mechanisms of action. This study provides our knowledge and understanding of platinum-based chemotherapy and potentially impacts future advancements in the treatment of cervical cancer and related diseases.