Whole-genome sequencing and analysis of the endophytic fungus Alternaria alternata Y-2 from Leymus chinensis

To analyze the auxiliary effect of endophytic fungus Alternaria alternata isolated from Leymus chinensis on the host plant at the genomic level, we isolated this strain of fungus, extracted the genomic DNA and sequenced the whole genome of A. alternata Y-2 on the Illumina Hiseq platform. The raw data was cleaned up using Trimmomatic and checked for quality using FastQC. The sequencing data was assembled using SPAdes, and GeneMark was used to perform gene prediction on the assembly results. The results showed that the genome size of A. alternata Y-2 was 37,383,676 bp, with 51% GC content, and the number of genes encoded was 12,724. A total of 90 tRNAs and 12 rRNAs were predicted. A total of 12627 genes were annotated in the NCBI nucleotide sequences Database, and 21 gene clusters were identifed. The annotation and functional analysis of the complete genome sequence of A. alternata Y-2 provide a theoretical basis for in-depth research on its future development and utilization.