Antimicrobial Resistance Patterns of Selected Gram-Negative Bacterial Pathogens Isolated from Clinical Samples in Rajshahi City, Bangladesh: A Cross-Sectional Study

In recent years, there has been a significant rise in the prevalence of multidrug resistance Acinetobacter, Pseudomonas, Escherichia coli and Salmonella spp in Bangladesh . Patients are most susceptible to infections like enteric fever, nosocomial, UTI and other infections. It is therefore subject of interest to observe the number of incidence and antimicrobial resistant pattern of Acinetobacter, Pseudomonas, Escherichia coli and Salmonella spp in patients. During 15 ^th November 2024 to 31 ^st December 2024. 250 samples (Blood and Throat swab) of enteric fever, nosocomial, UTI and others suspected patients were tested at Department of Microbiology, Rajshahi Medical College, Rajshahi. From samples cultures 144(57.6%) patients out of 250 patients were positive. The target patients were from both sexes and age in between <1 to 80 years, and highest isolates from the age between 51-70 years. Among the tested antibiotics Acinetobacter spp was (100%) resistant to Amikacin, Cefepime, Ceftazidime, Gentamycin, Ceftriaxone, Nitrofurantin, Rifampicin and Vancomycin, Azytromycin was (72.73%), Ciprofloxacin (63.64%), Cefixime (90.91%), Piperacillin (72.73%) and low level of Imipenem & Meropenem was 45.46%. High level of susceptible was recorded in Colistin (100%) and Imepenem & Meropenem (54.54 %). Pseudomonas spp was (100%) resistant to Gentamycin, Rifampicin & Cefepime, followed by Amikacin (90%), Nitrofurantoin (85%), Piperacillin (80%), Vancomycin (75%), Ceftriaxone (70%), Azithromycin (65%), Ciprofloxacin (60%), Ceftazidime (55%), Imipenem & Meropenem (20%). High level of susceptible was recorded in Colistin (100%) and Imepenem & Meropenem (80%). Escherichia coli was resistant to Gentamycin & Rifampicin 100%, Kanamycin 97.8%, Amikacin 94.6%, Cefepime & Vancomycin 93.5%, Nitrofurantoin 92.4%, Ceftriaxone, Piperacillin & Ceftazidime 88.1%, Cefixime 83.8%, Azithromycin 78.4%, Ciprofloxacin 77.4% and Imipenem & Meropenem 47.3%. It was susceptible to Colistin 100%, and Imipenem & Meropenem 52.7%. Salmonella spp. was resistant to Gentamycin, Rifampicin & Vancomycin (100%) followed by Azithromycin, Ceftazidime, Cefixime & nitrofurantoin (71.4%), Amikacin, Ciprofloxacin, Ceftazidime, Ceftriaxone & Piperacillin (57.1%) and sensitive to Colistin (100%), Imipenem & Meropenem (71.5%)