Soaking Up Success: Sponge-Assisted Nanoparticle Transfection
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Current cell transfection systems face efficiency limitations, particularly for challenging cell types. We present a sponge-assisted transfection method enabling efficient mRNA transfection at nanogram-scale doses—a several orders of magnitude-reduction compared to the microgram quantities typically required by lipid-based and membrane-disruption methods. This approach achieves robust, dose-dependent transfection across difficult-to-transfect cell types, establishing broad applicability for both basic research and therapeutic use.
