Assessment evaluation of ointments based on Lavandula angustifolia and Cymbopogon nardus on wound healing

The use of medicinal plants in veterinary medicine has increased in recent years, particularly in treating skin wounds. This trend is promising, mainly due to the phenomenon of resistance, which diminishes the effectiveness of certain conventional drugs. The objective was to develop ointments using volatiles from Lavandula angustifolia Mill. and Cymbopogon nardus (L.) Rendle and evaluate their effectiveness on surgical wounds in mice. Volatiles were analyzed by GC-MS, with the major as linalool (36.3%) and linalool acetate (30.4%) for L. angustifolia , and citronellal (45.8%) and geraniol (22.3%) for C. nardus . Ointments were produced at 2% and 5% concentrations, and physical-chemical tests were conducted to assess their stability. Animals were divided into five groups: G5-control, G1- L. angustifolia 5%, G2- C. nardus 5%, G3- L. angustifolia and C. nardus 2%, and G4-5%. Surgical injuries were performed, with daily treatments administered; on the 21st day, the animals were euthanized, and tissue samples were collected for histopathology analysis. The group treated with the 5% ointment containing L. angustifolia and C. nardus showed the most significant reduction in wound-healing time (82.5%), achieving it in 17 days, and exhibited no secretion or crusting during healing. Histological findings revealed mild recruitment of mononuclear cells, marked to moderate vascular proliferation, and organized collagen fibers of adequate intensity in this group. This research presents opportunities for further advances in the therapeutic properties of volatiles derived from medicinal plants.