Green synthesis of AgNPs using Rauvolfia tetraphylla leaf extract: Invitro antidiabetic potential and characterization using FTIR, UV-Vis spectrophotometry and SEM analysis

Diabetes mellitus is a serious health concern and the synthetic drugs used are effective with adverse effects. In this glimpse, plants become indispensable due to their distinctive physical, chemical and biological characteristics to counteract their negative effects. Rauvolfia tetraphylla leaves holds the essential phytochemical that plays crucial role in the management of diabetes. The present research aims to investigate the antioxidant capacity, antidiabetic property (α-amylase inhibition) using aqueous leaf extract and green synthesized silver nanoparticles. Metallic nanoparticle are essential in achieving target delivery of plant metabolite also improve insulin sensitivity. The silver nanoparticles were characterized by FT-IR, UV-Vis spectroscopy and SEM analysis. The aqueous leaf extract and biosynthesized AgNPs both displayed significant enzyme inhibition, and the AgNPs proved to be potent and more efficacious emphasizing their existence in disease management. FT-IR spectra revealed functional groups accountable for nanoparticle reduction and stabilization, and UV-Vis spectroscopy indicated absorption peaks at 230–250 nm and 350 nm. SEM images revealed rod-shaped and needle-like nanostructures with a crystalline morphology, indicates viable phytochemical-mediated synthesis. The increased bioactivity of AgNPs, resulting from their larger surface area and stabilization by plant biomolecules, suggests their potential as novel agents for treating diabetes and a potent antioxidant.