First Report of Rhizoctonia solani AG 2-2IIIB causing Damping-off on Bottle Gourd (Lagenaria siceraria) Seedlings in Bangladesh

  1. Md Ziaur Rahman Bhuiyan
  2. Mahmuda Akter
  3. Nazneen Sultana
  4. Md. Jahidul Islam
  5. Md. Akash Miah
  6. Md. Ferdous Alom
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Abstract

Bottle gourd is a popular winter vegetable crop in Bangladesh. Bottle gourd seedlings with damping off symptoms were collected in October 2024 from Savar upazila, Dhaka. The causal agent was grown in growth media, and studied the morphological features. Molecular identity was confirmed through amplification of internal-transcribed spacer (ITS) region, RNA polymerase II ( rpb2 ), and β-tubulin ( tub2 ) genes. Based on morphological and molecular investigations, the pathogen was verified as Rhizoctonia solani AG 2–2 IIIB. Pathogenicity test was done to fulfill the Koch’s postulates. These findings will be useful to design an effective disease management program of cucurbits in Bangladesh.

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  1. Version published to 10.21203/rs.3.rs-8263008/v1 on Research Square