The structural differences of intestinal flora between people with functional diarrhea and the general population

Objective To analyze the characteristics of intestinal flora in patients with Functional Diarrhe (FDr), explore the correlation between different bacterial genera and FDr, as well as its predictive and diagnostic value for FDr, and provide new ideas for the prevention and treatment of FDr. Method This study adopted a case-control design. Questionnaire surveys and fecal sampling analyses were conducted on patients with functional diarrhea and normal family members without gastroenteric-related diseases. The microbial diversity of fecal samples in each group was sequenced by 16S full-length rRNA using the third-generation Pacbio sequencing platform. Obtain DNA sequences and other data to conduct species difference analysis and study the association strength of each factor with the onset of FDr. Result A total of 64 samples were included, among which 32 were from the functional diarrhea group (case group) and 32 from the normal population group (control group).The results showed that the number of exposed pairs in the case group was significantly higher in terms of spicy diet and postmeal abdominal pain. Species composition analysis revealed that at the phylum level, the abundance ratio of actinomycetes in the case group increased, while that of Firmicutes decreased. At the genus level, the genus Bifidobacterium increased, while the genera Bacteroides and Faecalibacterium decreased. At the species level, Bifidobacterium pseudomonas was elevated, while Faecobacter procellarum was decreased. The diversity and uniformity of the case group decreased, while the richness and coverage of the intestinal flora in the two groups were basically the same. LefSe analysis screened out 41 differential bacterial genera. Among them, the relative abundance of Prevotella type 9, Prevotella Korella, etc. in the case group was higher than that in the control group, while the relative abundance of Bacteroides monocytoides, Clostridium difficile group 1, etc. was lower than that in the control group. Conclusion There are significant differences in the intestinal flora structure between the population with functional diarrhea and the normal population.