De novo chromosome-level genome assembly of two mycoparasitic hypocreaceous fungi

Mycoparasitic fungi in the Hypocreaceae family are ecologically and economically significant, yet genomic resources for many lineages remain limited. This is particularly true for the genus Hypomyces , which includes causative agents of cobweb disease in commercial mushrooms, and its putative relatives. Here, we present high-quality genome assemblies and annotations for two mycoparasitic Hypocreaceae fungi, Hypomyces semicircularis X01500 and a strain X01501 belonging to a putatively new species of Mycogone . Using a hybrid sequencing approach (Illumina and ONT), we generated highly contiguous assemblies for X01500 (31.45 Mb, 8 contigs, N50=4.92 Mb) and X01501 (37.64 Mb, 8 contigs, N50=5.66 Mb), with BUSCO completeness >99.7%. We annotated 11,121 and 9,717 protein-coding genes, respectively, with over 98.5% functionally characterized. A phylogenomic analysis confirmed the taxonomic identity of X01500 and supported X01501 as a distinct, novel lineage sister to Trichoderma . These genomes provide a crucial foundation for future research into the evolution and mechanisms of mycoparasitism within this understudied clade.